Canine Cortisol ELISA Kit (Colorimetric)

Canine Cortisol ELISA Kit (Colorimetric) [NBP3-23543] - Samples were spiked with high concentrations of Canine Cortisol and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Canine Cortisol ELISA Kit (Colorimetric) [NBP3-23543] - Standard Curve Reference

• Reactivity: Ca
• Applications: ELISA
• Suitable Sample Type: Serum and plasma
• Standard Curve Range: 3.13-200 ng/mL
• Sensitivity: 1.13 ng/mL

Canine Cortisol ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Canine Cortisol in samples. No significant cross-reactivity or interference between Canine Cortisol and analogues was observed.
• Standard Curve Range: 3.13-200 ng/mL
• Sensitivity: 1.13 ng/mL
• Assay Type: Competitive-ELISA
• Inter-Assay: %CV < 5.61
• Intra-Assay: %CV < 6.08
• Spike Recovery: 91-106%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Canine Cortisol ELISA Kit (Colorimetric)

• Compound F
• Cortisol
• Hydrocortisone

Background

Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.