Human Cortisol ELISA Kit (Colorimetric)

ELISA: Human Cortisol ELISA Kit (Colorimetric) [NBP3-18003] - Samples were spiked with high concentrations of Human Cortisol and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Cortisol ELISA Kit (Colorimetric) [NBP3-18003] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA, ELISA(Comp)
• Suitable Sample Type: Serum and Plasma
• Standard Curve Range: 6.25-400 ng/mL
• Sensitivity: 2.92 ng/mL

Human Cortisol ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 6.25-400 ng/mL
• Sensitivity: 2.92 ng/mL
• Assay Type: Competitive ELISA
• Inter-Assay: %CV < 8.96
• Intra-Assay: %CV < 8.78
• Spike Recovery: 81-100%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • Competitive ELISA
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Cortisol ELISA Kit (Colorimetric)

• Compound F
• Cortisol
• Hydrocortisone

Background

Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Cortisol ELISA Kit (NBP3-18003)(1)

Publication using NBP3-18003

• Calapai M, Puzzo L, Bova G et al. Effects of Physical Exercise and Motor Activity on Depression and Anxiety in Post?Mastectomy Pain Syndrome preprints.org 2023-11-23 [PMID: 38255692] (ELISA)

FAQs for Cortisol ELISA Kit (NBP3-18003). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.