Sheep Cortisol ELISA Kit (Colorimetric) Summary
| Description |
Cortisol (Sheep) ELISA Kit is used for the quantitative measurement of Cortisol in sheep serum or plasma. |
| Standard Curve Range |
1 to 80 ng/mL |
| Sensitivity |
0.1 ng/mL |
| Assay Type |
Quantification |
| Sample Volume |
40 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
| Dilutions |
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| Application Notes |
Useful for quantification. |
| Publications |
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Packaging, Storage & Formulations
Kit Components
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Components
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- 20X Wash concentrate
- Assay Diluent
- Cortisol Standard: 7 vials (ready to use)
- Enzyme conjugate (20X)
- Microwells coated with Cortisol MAb
- Stop Solution (ready to use)
- TMB Substrate (ready to use)
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Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Sheep Cortisol ELISA Kit (Colorimetric)
Background
Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
Publications for Cortisol ELISA Kit (KA0919)(1)
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Product General Protocols
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FAQs for Cortisol ELISA Kit (KA0919). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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