Cortisol Antibody

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Product Details

Summary
Reactivity AllSpecies Glossary
Applications ELISA
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
5.0 mg/ml

Order Details

Cortisol Antibody Summary

Immunogen
Cortisol-21-hemisuccinyl-KLH/BSA/Fetuin mixture.
Specificity
This product recognises the glucocorticoid hormone cortisol, produced in the adrenal cortex and involved in responses to stress.
The following cross reactivities have been observed:

Cortisol 100%
Corticosterone >90%
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
5.0 mg/ml
Purity
IgG purified

Alternate Names for Cortisol Antibody

  • Compound F
  • Cortisol
  • Hydrocortisone

Background

Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cortisol Antibody (NB100-62486) (0)

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Product General Protocols

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FAQs for Cortisol Antibody (NB100-62486). (Showing 1 - 1 of 1 FAQ).

  1.  I want to detect human free cortisol involved in saliva or urine by sandwich ELISA. Because you specified that immunogen of this product is cortisol-21-hemisuccinyl-KLH/BSA/Fetuin mixture, I want to know whether I can use this polyclonal cortisol antibody to recognize human free cortisol.
    • Our antibody NB100-62486 has been found to detect cortisol released from the adrenal cortex. We have not yet tested this antibody with saliva or urine samples, so we are not certain if the antibody will be sensitive enough for detection of this free cortisol. If you did wish to try this product in your experiment, we can offer you our Innovator's Reward Program since we have not yet tested this antibody with your particular samples. Our Innovator’s Reward™ program was created to allow researchers the opportunity to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply purchase the product as normal and submit an online review detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product to use in a future purchase of your choice. Reviews may be submitted through our distributors or by emailing innovators@novusbio.com.

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