Corticosterone ELISA Kit (Colorimetric)

ELISA: Corticosterone ELISA Kit [Biotin] [KA0468]

• Applications: ELISA, NULL
• Suitable Sample Type: Cell Culture Samples, Milk, Plasma, Saliva, Serum, Urine
• Standard Curve Range: 0.391 to 100 ng/mL
• Sensitivity: 0.29 ng/mL

Corticosterone ELISA Kit (Colorimetric) Summary

• Description: Corticosterone ELISA Kit is a competitive enzyme immunoassay for the quantitative measurement of corticosterone.
• Standard Curve Range: 0.391 to 100 ng/mL
• Sensitivity: 0.29 ng/mL
• Assay Type: Competitive ELISA
• Inter-Assay: CV% < 7.3%
• Intra-Assay: CV% < 4.8%
• Sample Volume: 25 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Corticosterone ELISA Kit (Colorimetric)

• 17-Deoxycortisol;C21H30O4
• Corticosterone

Background

Anti-corticosterone is used to test Corticosterone Levels. In Men levels are usually 420 - 220 pg/0.1 ml2. For Women in the Follicular Phase levels are 240 - 150 pg/0.1 ml and 440 - 230 pg/0.1 ml3 in the luteal phase. For laying Hens corticosterone levels are 220 - 50 pg/0.1 ml.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Corticosterone ELISA Kit (KA0468)(34)

Publications using KA0468

• Wen-Hsin L, Hsu-Wen C, Pei-Yi L et al. CPEB3-dowregulated Nr3c1 mRNA translation confers resilience to developing posttraumatic stress disorder-like behavior in fear-conditioned mice. Neuropsychopharmacology. 2021-05-03 [PMID: 33941859]
• Mees LM, Coulter MM, Chrenek MA et al. Low-Intensity Exercise in Mice Is Sufficient to Protect Retinal Function During Light-Induced Retinal Degeneration. Invest Ophthalmol Vis Sci. 2019-04-01 [PMID: 30933260]
• Ziko I, Sominsky L, De Luca SN et al. Acylated ghrelin suppresses the cytokine response to lipopolysaccharide and does so independently of the hypothalamic-pituitary-adrenal axis. Brain Behav Immun 2018-07-17 [PMID: 30009998]
• Zubeldia-Plazaola A, Recalde-Percaz L, Moragas N et al. Glucocorticoids promote transition of ductal carcinoma in situ to invasive ductal carcinoma by inducing myoepithelial cell apoptosis. Breast Cancer Res 2018-07-04 [PMID: 29973218]
• Reichenbach A, Stark R, Mequinion M et al. Carnitine acetyltransferase (Crat) in hunger-sensing AgRP neurons permits adaptation to calorie restriction. FASEB J 2018-06-22 [PMID: 29932868]
• Reichenbach A, Mequinion M, Bayliss JA et al. Carnitine acetyltransferase in Agrp neurons is required for the homeostatic adaptation to restricted feeding in male mice. Endocrinology 2018-04-24 [PMID: 29697769]
• Christiansen SL, Hoojgaard K, Wiborg O et al. Disturbed diurnal rhythm of three classical phase markers in the chronic mild stress rat model of depression. Neuroscience Resarch Mar 23 2016
• Pitta S, Augustine BB, Kasala ER et al. Honokiol reverses depressive-like behavior and decrease in brain BDNF levels induced by chronic corticosterone injections in mice. Pharmacognosy Journal 2013-11-07 (ELISA, Mouse)
• Chen Q, Yan Z, Zhang H et al. Role of Nanocrystallites of Al-Based Glasses and H 2 O 2 in Degradation Azo Dyes Materials 2020-12-24 [PMID: 33374210] (ELISA)
• Tsukamoto M, Yamashita M, Nishi T et Al. A Human ABC Transporter ABCC4 Gene SNP (rs11568658, 559 G > T, G187W) Reduces ABCC4-Dependent Drug Resistance Cells 2019-01-10 [PMID: 30634695] (ELISA)
• Hervig ME, Jensen NC, Rasmussen NB et al. Involvement of serotonin 2A receptor activation in modulating medial prefrontal cortex and amygdala neuronal activation during novelty-exposure. Behav Brain Res 2017-03-02 [PMID: 28263831]
• Xiao Y, Xia T, Yu J et al. Knockout of inositol-requiring enzyme 1alpha in pro-opiomelanocortin neurons decreases fat mass via increasing energy expenditure. Open Biol. 2016-08-01 [PMID: 27558934] (ELISA, Mouse)
• Stark R, Santos VV, Geenen B et al. Des-acyl ghrelin and Ghrelin O-Acyltransferase regulate Hypothalamic-Pituitary-Adrenal axis activation and anxiety in response to acute stress. Endocrinology 2016-08-04 [PMID: 27490185]
• Christiansen SL, Hoojgaard K, Wiborg O, Bouzinova EV. Disturbed diurnal rhythm of three classical phase markers in the chronic mild stress rat model of depression. Neuroscience Resarch 2016-03-23 [PMID: 27033803]
• Sulakhiya K, Keshavlal GP, Bezbaruah BB et al. Lipopolysaccharide induced anxiety- and depressive-like behaviour in mice are prevented by chronic pre-treatment of esculetin. Neuroscience Letters. 2015-11-24 [PMID: 26620836]
• Ye L, Hu Z, Wang H et al. Tris-(2,3-Dibromopropyl) Isocyanurate, a New Emerging Pollutant, Impairs Cognition and Provokes Depression-Like Behaviors in Adult Rats. PLoS One 2015-10-12 [PMID: 26458255]
• Sulakhiya K, Kumar P, Jangra A et al. Honokiol abrogates lipopolysaccharide-induced depressive like behavior by impeding neuroinflammation and oxido-nitrosative stress in mice. Eur J Pharmacol. 2014-10-14 [PMID: 25446914]
• Lee CY. Adenosine protects Sprague Dawley rats from high-fat diet and repeated acute restraint stress-induced intestinal inflammation and altered expression of nutrient transporters. J Anim Physiol Anim Nutr (Berl). 2014-09-06 [PMID: 25196093]
• Long L, Toda C, Jeong JK et al. PPARgamma ablation sensitizes proopiomelanocortin neurons to leptin during high-fat feeding. J Clin Invest. 2014-08-01 [PMID: 25083994]
• Lin PY, Chang AY, Lin TK. Simvastatin treatment exerts antidepressant-like effect in rats exposed to chronic mild stress. Pharmacol Biochem Behav. 2014-06-07 [PMID: 24931338]
• Kenny R, Dinan T, Cai G, Spencer SJ. Effects of mild calorie restriction on anxiety and hypothalamic-pituitary-adrenal axis responses to stress in the male rat. Physiol Rep. 2014-03-01 [PMID: 24760519]
• Murthy KD, George MC, Ramasamy P, Mustapha ZA. Housing under the pyramid reduces susceptibility of hippocampal CA3 pyramidal neurons to prenatal stress in the developing rat offspring. Indian Journal of Experimental Biology Vol. 51. 2013-12-01 [PMID: 24579372]
• Hansen RT 3rd, Conti M, Zhang HT. Mice deficient in phosphodiesterase-4A display anxiogenic-like behavior. Psychopharmacology (Berl). 2014-08-01 [PMID: 24563185] (Mouse)
• Martelli D, Yao ST, McKinley MJ, McAllen RM. Reflex control of inflammation by sympathetic nerves, not the vagus. J Physiol. 2014-01-13 [PMID: 24421357]
• Pitts MW, Reeves MA, Hashimoto AC et al. Deletion of Selenoprotein M Leads to Obesity Without Cognitive Deficits. J Biol Chem. 2013-07-23 [PMID: 23880772]
• Colaianna M, Schiavone S, Zotti M et al. Neuroendocrine profile in a rat model of psychosocial stress: relation to oxidative stress. Antioxid Redox Signal. 2013-01-16 [PMID: 23320850]
• Spencer SJ, Xu L, Clarke MA et al. Ghrelin Regulates the Hypothalamic-Pituitary-Adrenal Axis and Restricts Anxiety After Acute Stress. Biol Psychiatry. 2012-04-20 [PMID: 22521145]
• Palmer NO, Bakos HW, Owens JA et al. Diet and exercise in an obese mouse fed a high-fat diet improve metabolic health and reverse perturbed sperm function. Am J Physiol Endocrinol Metab. 2012-04-01 [PMID: 22252945] (Mouse)
• Steyn FJ, Leong JW, Huang L et al. GH does not modulate the early fasting-induced release of free fatty acids in mice. Endocrinology. 2012-01-01 [PMID: 22109889]
• Howell KR, Kutiyanawalla A, Pillai A. Long-term continuous corticosterone treatment decreases VEGF receptor-2 expression in frontal cortex. PLoS One. 2011-05-27 [PMID: 21647420]
• Bulfin LJ, Clarke MA, Buller KM, Spencer SJ. Anxiety and hypothalamic-pituitary-adrenal axis responses to psychological stress are attenuated in male rats made lean by large litter rearing. Psychoneuroendocrinology. 2011-02-22 [PMID: 21349647]
• Sheppard KE. Corticosteroid receptors, 11 beta-hydroxysteroid dehydrogenase, and the heart. Vitam Horm. 2003-01-01 [PMID: 12852253]
• Schaaf MJ, De Kloet ER, Vreugdenhil E. Corticosterone effects on BDNF expression in the hippocampus. Implications for memory formation. Stress. 2000-05-01 [PMID: 10938581]
• Herbert J. Neurosteroids, brain damage, and mental illness. Exp Gerontol. 1998-11-01 [PMID: 9951618]

FAQs for Corticosterone ELISA Kit (KA0468). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.