Concanavalin A [Cy3]

Immunofluorescence: Concanavalin A [Cy3] [CL-1003-NB] - Tonsil FFPE antigen retrieved and stained with Cy3 ConA (red-orange). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.

Concanavalin A [Cy3] [CL-1003-NB] - Concanavalin A (Con A) Cy3

Product Discontinued

Concanavalin A [Cy3] Summary

• Description: Features:
  
  
• Con A recognizes alpha-linked mannose present as part of a core oligosaccharide in many serum and membrane glycoproteins.
• Cy3 labeled Con A has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin.
• This conjugate is supplied essentially free of unconjugated fluorochromes.
• Excitation: 552 nm
• Emission: 565 nm
• Color: Red

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: A precipitate may form during storage. This does not have a significant adverse effect on the product. If a precipitate forms upon long-term storage at 4C, warm to 37C and centrifuge before use. The recommended concentration range for use is 5-20 ug/mL. .

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.1 mM CaCl2, proprietary stabilizer
• Preservative: 0.08% Sodium Azide

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

Con A recognizes alpha-linked mannose present as part of a core oligosaccharide in many serum and membrane glycoproteins. At neutral and alkaline pH, Con A exists as a tetramer of four identical subunits; below pH 5.6, Con A dissociates into active dimers of 52 kDa. Acetylation, succinylation, or other derivatizations can also produce stable forms with dimeric structures. Nicks in the sequence are often present in the purest preparations due to hydrolytic damage within the seeds. Con A requires calcium or manganese ions at each of its four saccharide binding sites. Although these divalent metal ions are bound tightly to the polypeptide structure, buffers which can bind calcium (such as phosphate) generally should be avoided in diluting Con A, since a gradual loss in activity may occur.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.