Human Collagen Type I ELISA Kit (Colorimetric)

ELISA: Human Collagen Type I ELISA Kit (Colorimetric) [NBP2-30102]

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (example only; lot dependent)
• Standard Curve Range: 0.156-10 ng/mL (example only; lot dependent)
• Sensitivity: 0.059 ng/mL (example only; lot dependent)

Human Collagen Type I ELISA Kit (Colorimetric) Summary

• Description: Collagen Type I (COL1) ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL1 in human serum, plasma, tissue homogenates and other biological fluids.
• Specificity: The positive control is from a human serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly. The human based material used in this product is screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.
  
  Assay Procedure Summary
  1. Prepare all reagents, samples and standards.
  2. Add 100ul standard or sample to each well. Incubate 1 hour at 37C.
  3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C.
  4. Aspirate and wash 3 times.
  5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C.
  6. Aspirate and wash 5 times.
  7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37C.
  8. Add 50ul Stop Solution. Read at 450 nm immediately.
• Standard Curve Range: 0.156-10 ng/mL (example only; lot dependent)
• Sensitivity: 0.059 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL (example only; lot dependent)
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage is content dependent.

Notes

The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Dissolve with 150ul standard diluent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20C. After reconstitution, store Positive Control vial at 4C and use within 5 days.

Alternate Names for Human Collagen Type I ELISA Kit (Colorimetric)

• Collagen I
• Collagen Type 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Collagen Type I ELISA Kit (NBP2-30102)(1)

Publication using NBP2-30102

• Rakshit M, Gautam A, Toh L et al. Hydroxyapatite Particles Induced Modulation of Collagen Expression and Secretion in Primary Human Dermal Fibroblasts IJN 2020-07-01 [PMID: 32764927] (ELISA, Human)