Clostridium Difficile Toxin A Antibody (PCG4.1)

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Product Details

Summary
Reactivity Ba, HaSpecies Glossary
Applications WB, ELISA
Clone
PCG4.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.16 mg/ml

Order Details

Clostridium Difficile Toxin A Antibody (PCG4.1) Summary

Immunogen
Full length protein A (C. difficile).
Localization
Secreted
Specificity
C. difficile Toxin A. Does not cross react with C. difficile Toxin B
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
Application Notes
Works with stool samples.
Publications
Read Publications using
NB600-1066 in the following applications:

  • 2 publications
  • WB
    7 publications

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 27647869).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
10mM Sodium Phosphate (pH 7.4) and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.16 mg/ml
Purity
Protein A purified

Alternate Names for Clostridium Difficile Toxin A Antibody (PCG4.1)

  • TcdA
  • ToxA
  • Toxin A

Background

Clostridium difficile, or C. difficile, a gram positive anaerobic bacterium, is the major causative agent of colitis and diarrhoea that can occur following antibiotic intake. C. difficile infection is one of the most common hospital acquired infections. When C. difficile colonize the gut, they release two potent toxins, toxin A and toxin B, which bind to certain receptors in the lining of the colon and ultimately cause diarrhoea and inflammation of the large intestine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Clostridium Difficile Toxin A Antibody (NB600-1066)(11)

We have publications tested in 2 confirmed species: Bacteria, Hamster.

We have publications tested in 2 applications: ELISA, WB.


Filter By Application
ELISA
(2)
WB
(7)
All Applications
Filter By Species
Bacteria
(7)
Hamster
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB600-1066 Applications Species
Kroh HK, Chandrasekaran R, Rosenthal K et al. Use of a neutralizing antibody helps identify structural features critical for binding of Clostridium difficile toxin TcdA to the host cell surface J. Biol. Chem. 2017 Jul 13 [PMID: 28705932] (Bacteria) Bacteria
Anjuwon-Foster BR, Tamayo R. A genetic switch controls the production of flagella and toxins in Clostridium difficile. PLoS Genet. Mar 27 2017 [PMID: 28346491] (WB, Bacteria) WB Bacteria
Childress KO, Edwards AN, Nawrocki KL et al. The Phosphotransfer Protein CD1492 Represses Sporulation Initiation in Clostridium difficile. Infect. Immun. Dec 1 2016 [PMID: 27647869] (WB, Hamster) WB Hamster
Edwards AN, Tamayo R, McBride SM. A Novel Regulator Controls Clostridium difficile Sporulation, Motility and Toxin Production. Mol. Microbiol. 2016 Feb 24 [PMID: 26915493] (WB, Bacteria) WB Bacteria
Willing SE, Richards EJ, Sempere L et al. Increased toxin expression in a Clostridium difficile mfd mutant. BMC Microbiol. 2015 Dec 18 [PMID: 26679502] (WB, Bacteria)

Details:
Clostridium Difficile Toxin A antibody (clone PCG4.1) was used for WB assays in experiments involving Clostridium difficile mfd mutant. The antibody was used at 1:1000 dilution in 3 % milk powder and detected using HRP-conjugated goat anti-mouse secondary antibody.
WB Bacteria
McKee RW, Mangalea MR, Purcell EB et al. The Second Messenger Cyclic Di-GMP Regulates Clostridium difficile Toxin Production by Controlling Expression of sigD. J Bacteriol. 2013 Nov [PMID: 24039264] (WB, Bacteria) WB Bacteria
Kolling GL, Wu M, Alcantara-Warren C et al. Lactic acid production by Streptococcus thermophilus alters Clostridium difficile infection and in vitro Toxin A production Gut Microbes 2012 Nov 1 [PMID: 22895082] (WB, Bacteria) WB Bacteria
Aubry A, Hussack G, Chen W et al. Modulation of Toxin Production by the Flagellar Regulon in Clostridium difficile Infect Immun 2012 Oct [PMID: 22851750] (ELISA, WB, Bacteria) ELISA, WB Bacteria
Vohra P, Poxton IR. Comparison of toxin and spore production in clinically relevant strains of Clostridium difficile. Microbiology;157(Pt 5):1343-53. 2011 May. [PMID: 21330434] (ELISA) ELISA
Goulding D, Thompson H, Emerson J et al. Distinctive profiles of infection and pathology in hamsters infected with Clostridium difficile strains 630 and B1. Infect Immun;77(12):5478-85. 2009 Dec. [PMID: 19752031]
Show All 11 Publications.

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Product General Protocols

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Video Protocols

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FAQs for Clostridium Difficile Toxin A Antibody (NB600-1066). (Showing 1 - 3 of 3 FAQs).

  1. I am seeking monoclonal antibodies specific for the C Terminal binding regions of clostridum difficile toxins A and B (aa1852-2366)?
    • I would recommend catalog # NB100-62473. Although it is a polyclonal, it is the only one that we know binds to the C-Terminal portion of the protein. The other antibodies we have against both targets are synthesized from the full length sequence. We do not routinely epitope map our antibodies so we cannot be certain where they are binding. This polyclonal is synthesized against a small 10 amino acid region of the protein so it should be highly specific to that location.
  2. Was NB-600-1066 Clostridium Difficile Toxin A antibody PCG4.1 purified from ascites or tissue culture supernatant? We are in search of an ELISA grade anti- Tcd A antibody that does not contain other antibodies.
    • Our Clostridium Difficile Toxin A Antibody catalog number NB600-1066 was protein A purified from mouse ascites.
  3. Two questions: 1. Do you have any available data regarding the titer of NB600-1066, Anti C. difficile toxin A mAB PGC4 in ELISA? 2. I see that you sell other C. difficile toxin A and B antibodies. Is there any available ELISA data on those antibodies? And are they free from other nonspecific antibodies? Or are all of your mABs purified from ascites?
    • 1. We do not have an in-house ELISA titer, but I have two publications that have used NB600-1066 in ELISA applications: PMID 21330434 and PMID 22851750. 2. For our other Clostridium Difficile products, the ELISA testing data came from either publications or from the clone inventors. We unfortunately do not have any in-house ELISA data for these products. Our mABs can be purified from either tissue culture supernatant or ascites. We try to include the source on our datasheet, but some of them are missing this information. If you have a specific product, we can always look this information up for you. Since our mABs are either sold unpurified or protein A/G purified, there is a possibility for some non-specific antibodies to be present. We unfortunately do not test our products for the presence of other antibodies.

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