Clostridium Botulinum Toxin A Antibody (2A33)

Product Discontinued

Clostridium Botulinum Toxin A Antibody (2A33) Summary

• Immunogen: amino acid region 869-887, C. botulinum toxin A heavy chain
• Specificity: Mouse Monoclonal Clostridium botulinum toxin A (2A33)
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Purity: IgM purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: IgM purified

Alternate Names for Clostridium Botulinum Toxin A Antibody (2A33)

• Atx
• Bna
• BoNT/A
• Bontoxilysin A
• BotA
• BOTOX
• Botulinum neurotoxin type A
• Botulism A toxoid

Background

Clostridium botulinum neurotoxins are zinc-dependent proteases that block the release of acetylcholine from peripheral cholinergic nerve endings by targeting specific membrane proteins including synaptosomal-associated proteins (SNAP), vesicle-associated membrane proteins (VAMP), and syntaxins. Neurotoxin A is characteristically associated with human botulism and known to target and hydrolyse the 197-Gln-|-Arg-198 bond of SNAP-25.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Clostridium Botulinum Toxin A Antibody (NB100-73115). (Showing 1 - 1 of 1 FAQ).

1. We would like to know LOD (limit of detection) numerical value of NB100-73115.
   • NB100-73115 - the Limit of Detection we have found using direct ELISA is greater than 1:10,000 with no amplification using OPD (o-phenylenediamine dihydrochloride) development and HRP 2nd antibody. Please remember, each end user needs to optimize the use of this product in their own application.