Clostridium Botulinum Toxin A Antibody (2A33)

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Product Details

Summary
Reactivity BaSpecies Glossary
Applications WB, ELISA
Clone
2A33
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Clostridium Botulinum Toxin A Antibody (2A33) Summary

Immunogen
amino acid region 869-887, C. botulinum toxin A heavy chain
Specificity
Mouse Monoclonal Clostridium botulinum toxin A (2A33)
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Purity
IgM purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Purity
IgM purified

Alternate Names for Clostridium Botulinum Toxin A Antibody (2A33)

  • Atx
  • Bna
  • BoNT/A
  • Bontoxilysin A
  • BotA
  • BOTOX
  • Botulinum neurotoxin type A
  • Botulism A toxoid

Background

Clostridium botulinum neurotoxins are zinc-dependent proteases that block the release of acetylcholine from peripheral cholinergic nerve endings by targeting specific membrane proteins including synaptosomal-associated proteins (SNAP), vesicle-associated membrane proteins (VAMP), and syntaxins. Neurotoxin A is characteristically associated with human botulism and known to target and hydrolyse the 197-Gln-|-Arg-198 bond of SNAP-25.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Clostridium Botulinum Toxin A Antibody (NB100-73115). (Showing 1 - 1 of 1 FAQ).

  1. We would like to know LOD (limit of detection) numerical value of NB100-73115.
    • NB100-73115 - the Limit of Detection we have found using direct ELISA is greater than 1:10,000 with no amplification using OPD (o-phenylenediamine dihydrochloride) development and HRP 2nd antibody. Please remember, each end user needs to optimize the use of this product in their own application.

Secondary Antibodies

 

Isotype Controls

Additional Clostridium Botulinum Toxin A Products

Clostridium Botulinum Toxin A NB100-73115

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