Chromata ChIP Histone H3 [ac Lys9, ac Lys14] ChIP Kit

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Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [ac Lys9, ac Lys14] ChIP Kit [NBP1-71714] - 2 ug of NB21-1023 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by ...read more
Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [ac Lys9, ac Lys14] Kit [NBP1-71714] - See notes for protocol information on ChromataChIP kits.
Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [ac Lys9, ac Lys14] ChIP Kit [NBP1-71714] - qRT-PCR standard curve generated from a serial dilution of sheared HeLa chromatin.
Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [ac Lys9, ac Lys14] ChIP Kit [NBP1-71714] - PCR product melt curve of 1% chromatin immunoprecipitation input sample run in duplicate.

Product Details

Summary
Applications ChIP

Order Details

Chromata ChIP Histone H3 [ac Lys9, ac Lys14] ChIP Kit Summary

Description
This ChIP kit is specific for Histone H3 acetylated Lysine 9/Lysine 14, and contains all the necessary components to perform 25 Immunoprecipitation experiments and a positive control antibody (NB21-1023SS). This ChIP kit is a complete pack to help you perfom Chromatin Immunoprecipitation efficiently and accurately.

This kit comes with a full size vial of acetyl Lys9/Lys14 antibody (NB21-1081).
Modification
ac Lys9, ac Lys14
Kit Type
ChIP Kit

Applications/Dilutions

Publications
Read Publications using NBP1-71714.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Purification Columns (30 EA)
  2. Proteinase K (0.06 mL)
  3. Protein A/G Magnetic Beads (0.8 mL)
  4. H3 K4Me3 Positive Control Antibody (0.025 mg) - NB21-1023SS
  5. H3 acetyl Lys9/Lys14 Antibody (0.05 mg) - NB21-1081
  6. DNA Wash Buffer (5 mL - add 20 mL EtOH)
  7. DNA Binding Buffer (18 mL)
  8. DNA Elution Buffer (2 mL)
  9. Human RPL30 Positive Control Primer Pair at 10 uM (0.5 mL) - NBP1-71651
  10. Human AFM Negative Control Primer Pair at 10 uM (0.5 mL) - NBP1-71653
  11. IP Dilution Buffer (2 x 25 mL)
  12. IP Wash Buffer 3 (15 mL)
  13. IP Wash Buffer 2 (15 mL)
  14. IP Wash Buffer 1 (15 mL)
  15. IP Elution Buffer (7 mL)
  16. IP Wash Buffer 4 (15 mL)
  17. 10X Glycine (30 mL)
  18. 5M NaCl (1 mL)

Notes

Click here for detailed information on the ChromataChIP kit. View the complete ChromatChIP Kit components and protocol here.

Alternate Names for Chromata ChIP Histone H3 [ac Lys9, ac Lys14] ChIP Kit

  • H3K14ac
  • H3K9ac
  • H3K9acK14ac

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Chromata ChIP Histone H3 Kit (NBP1-71714)(2)

We have publications tested in 1 application: ChIP.


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Showing Publications 1 - 2 of 2.
Publications using NBP1-71714 Applications Species
Paul B Noto, Yuri Bukhtiyarov, Meng Shi et al. Regulation of Sphingomyelin Phosphodiesterase, Acid-like 3A Gene (SMPDL3A) by Liver X Receptors. Molecular Pharmacology 2012 July 24 [PMID: 22810003] (ChIP) ChIP
Fishman J, Homon A, Lamsa E et al. The Complete Chemical Synthesis of Histones H3 and H4 Containing Epigenetic Modifications and Their Use in Characterizing Arginine Methylated Histone Antibodies. Poster presented at ASCB. 21st Century Biochemicals, Marlborough, MA 01752, Novus Biologicals, LLC, 8100 Southpark Way, Littleton, CO 80120.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ChIP Webinar
ChIP Video Protocol

FAQs for Chromata ChIP Histone H3 Kit (NBP1-71714). (Showing 1 - 1 of 1 FAQs).

  1. I have one question about ChIP. In your Chromata ChIP kit, you add 25ul magnetic beads to the samples. Is there any way to normalize for differences in the amounts of magnetic beads in each sample? For example, it can happen that one sample got a bit more beads than another, is there some way to take this into account when you analyze your data?
    • As long as the vial/bottle of beads is vortexed so that the beads are fully resuspended in solution before taking the 25ul out, there will not be any significant variation in bead number. However, if you are taking multiple 25ul aliquots out, it is best to quickly revortex before every aliquot pipetting. For all of our internal testing, we typically see a CV of less than 2% for both intra- and interassay variation, which is very good.

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