Chromata ChIP Histone H3 [Monomethyl Lys9] ChIP Kit

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Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [Monomethyl Lys9] Kit [NBP1-71713] - See notes for protocol information on ChromataChIP kits.
Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [Monomethyl Lys9] Kit [NBP1-71713] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.
Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [Monomethyl Lys9] Kit [NBP1-71713] - PCR product melt curve of 1% chromatin immunoprecipitation input sample run in duplicate.
Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [Monomethyl Lys9] Kit [NBP1-71713] - 2 ug of NB21-1023 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by ...read more

Product Details

Summary
Applications ChIP

Order Details

Chromata ChIP Histone H3 [Monomethyl Lys9] ChIP Kit Summary

Description
This ChIP kit is specific for Histone H3 monomethyl Lysine 9, and contains all the necessary components to perform 25 Immunoprecipitation experiments and a positive control antibody (NB21-1023SS).
Modification
Monomethyl Lys9
Specificity
This ChIP kit is specific for Histone H3 monomethyl Lysine 9, and contains all the necessary components to perform 25 Immunoprecipitation experiments and a positive control antibody (NB21-1023SS).
Kit Type
ChIP Kit

Applications/Dilutions

Application Notes
This ChIP kit is a useful tool for Chromatin Immunoprecipitation.
Publications
Read Publications using NBP1-71713.

Packaging, Storage & Formulations

Storage
Storage is content dependent.
Buffer
Dependent on kit component.

Kit Components

Components
  1. Proteinase K
  2. Purification Column Tubes
  3. Purification Columns
  4. 10x Glycine
  5. 5M NaCl
  6. DNA Wash Buffer
  7. DNA Binding Buffer
  8. DNA Elution Buffer
  9. H3 K4Me3 Positive Control Antibody
  10. Protein A/G Magnetic Beads
  11. IP Wash Buffer 3
  12. IP Wash Buffer 4
  13. IP Elution Buffer
  14. IP Dilution Buffer
  15. IP Wash Buffer 1
  16. IP Wash Buffer 2
  17. Human RPL30 Positive Control Primer Pair

Notes

Click here for detailed information on the ChromataChIP kit View the complete ChromatChIP Kit components and protocol here.

In addition, this kit comes with a full size vial of Monomethyl Lys9 antibody (NB21-1071).

Alternate Names for Chromata ChIP Histone H3 [Monomethyl Lys9] ChIP Kit

  • H3K9Me1

Background

This ChIP kit is a complete pack to help you perfom Chromatin Immunoprecipitation efficiently and accurately in your laboratory. We've taken all the guesswork out of ChIP by testing multiple component options to find the best possible component for you, the customer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Chromata ChIP Histone H3 Kit (NBP1-71713)(2)

We have publications tested in 1 application: ChIP.


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Showing Publications 1 - 2 of 2.
Publications using NBP1-71713 Applications Species
Paul B Noto, Yuri Bukhtiyarov, Meng Shi et al. Regulation of Sphingomyelin Phosphodiesterase, Acid-like 3A Gene (SMPDL3A) by Liver X Receptors. Molecular Pharmacology 2012 July 24 [PMID: 22810003] (ChIP) ChIP
Fishman J, Homon A, Lamsa E et al. The Complete Chemical Synthesis of Histones H3 and H4 Containing Epigenetic Modifications and Their Use in Characterizing Arginine Methylated Histone Antibodies. Poster presented at ASCB. 21st Century Biochemicals, Marlborough, MA 01752, Novus Biologicals, LLC, 8100 Southpark Way, Littleton, CO 80120.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ChIP Video Protocol
ChIP Webinar

FAQs for Chromata ChIP Histone H3 Kit (NBP1-71713). (Showing 1 - 1 of 1 FAQs).

  1. I have one question about ChIP. In your Chromata ChIP kit, you add 25ul magnetic beads to the samples. Is there any way to normalize for differences in the amounts of magnetic beads in each sample? For example, it can happen that one sample got a bit more beads than another, is there some way to take this into account when you analyze your data?
    • As long as the vial/bottle of beads is vortexed so that the beads are fully resuspended in solution before taking the 25ul out, there will not be any significant variation in bead number. However, if you are taking multiple 25ul aliquots out, it is best to quickly revortex before every aliquot pipetting. For all of our internal testing, we typically see a CV of less than 2% for both intra- and interassay variation, which is very good.

Additional Chromata ChIP Histone H3 Products

Chromata ChIP Histone H3 NBP1-71713

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