Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [ac Lys4] Kit [NBP1-71710] - 2 ug of NB21-1023 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and ...read more
Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [ac Lys4] Kit [NBP1-71710] - See notes for protocol information on ChromataChIP kits.
Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [ac Lys4] Kit [NBP1-71710] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.
Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [ac Lys4] Kit [NBP1-71710] - PCR product melt curve of 1% chromatin immunoprecipitation input sample run in duplicate.
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Click here for detailed information on the ChromataChIP kit View the complete ChromatChIP Kit components and protocol here.
In addition, this kit comes with a full size vial of acetyl Lys4 antibody (NB21-1024).
This ChIP kit is a complete pack to help you perfom Chromatin Immunoprecipitation efficiently and accurately in your laboratory. We've taken all the guesswork out of ChIP by testing multiple component options to find the best possible component for you, the customer.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
Publications for Chromata ChIP Histone H3 Kit (NBP1-71710)(2)
We have publications tested in 1 application: ChIP.
Fishman J, Homon A, Lamsa E et al. The Complete Chemical Synthesis of Histones H3 and H4 Containing Epigenetic Modifications and Their Use in Characterizing Arginine Methylated Histone Antibodies. Poster presented at ASCB. 21st Century Biochemicals, Marlborough, MA 01752, Novus Biologicals, LLC, 8100 Southpark Way, Littleton, CO 80120.
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Product General Protocols
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FAQs for Chromata ChIP Histone H3 Kit (NBP1-71710). (Showing 1 - 1 of 1 FAQs).
I have one question about ChIP. In your Chromata ChIP kit, you add 25ul magnetic beads to the samples. Is there any way to normalize for differences in the amounts of magnetic beads in each sample? For example, it can happen that one sample got a bit more beads than another, is there some way to take this into account when you analyze your data?
As long as the vial/bottle of beads is vortexed so that the beads are fully resuspended in solution before taking the 25ul out, there will not be any significant variation in bead number. However, if you are taking multiple 25ul aliquots out, it is best to quickly revortex before every aliquot pipetting. For all of our internal testing, we typically see a CV of less than 2% for both intra- and interassay variation, which is very good.