Monkey Chorionic Gonadotropin beta Chain (hCG beta) ELISA Kit (Colorimetric)

ELISA: Monkey Chorionic Gonadotropin beta Chain (hCG beta) ELISA Kit (Colorimetric) [NBP2-75263] - Samples were spiked with high concentrations of Monkey Chorionic Gonadotropin beta Chain (hCG beta) and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Monkey Chorionic Gonadotropin beta Chain (hCG beta) ELISA Kit (Colorimetric) [NBP2-75263] - Standard Curve Reference

• Reactivity: Pm
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL

Monkey Chorionic Gonadotropin beta Chain (hCG beta) ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Monkey CG(Beta) in samples. No significant cross-reactivity or interference between Monkey CG(Beta) and analogues was observed.
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.65%
• Intra-Assay: CV% < 5.39%
• Spike Recovery: 87-109%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CGB3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Monkey Chorionic Gonadotropin beta Chain (hCG beta) ELISA Kit (Colorimetric)

• CGB
• CGB3
• CGB3choriogonadotropin subunit beta
• CGB5
• CGB7
• CGB8
• CG-Beta
• Choriogonadotropin Subunit Beta
• Chorionic gonadotrophin chain beta
• chorionic gonadotropin beta 3 subunit
• Chorionic Gonadotropin beta Chain
• chorionic gonadotropin beta subunit
• chorionic gonadotropin, beta polypeptide
• hCGB

Background

Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Chorionic Gonadotropin beta Chain (hCG beta) ELISA Kit (NBP2-75263). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CGB3