Choline Oxidase Antibody

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Western Blot: Choline Oxidase Antibody [NB600-866] - Samples of 1 and 0.25 ug of purified Choline Oxidase per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples of purified Choline ...read more
Western Blot: Choline Oxidase Antibody [NB600-866] - Analysis using the Biotin conjugate of NB600-866. Detection of Lane 1: Choline Oxidase (Alcaligenes species). Load: 50 ng per lane. Primary antibody: Goat ...read more
Western Blot: Choline Oxidase Antibody [NB600-866] - Analysis using the HRP conjugate of NB600-866. Detection of antibody with Bilirubin Oxidase under reducing (R) conditions. A samples of ~1ug of purified protein ...read more
Western Blot: Choline Oxidase Antibody [NB600-866] - Analysis using the HRP conjugate of NB600-866. Detection of Lane 1: Bilirubin Oxidase (Myrothecium verrucaria). Load: 50 ng per lane. Primary antibody: Goat ...read more
ELISA: Choline Oxidase Antibody [NB600-866] - Analysis using Biotin conjugate of NB600-866. Detection of Choline Oxidase. Samples of 1 and 0.25 ug of purified Choline Oxidase per lane were run by SDS-PAGE under reducing ...read more

Product Details

Summary
Reactivity BaSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

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Catalog# & Conjugate Size Price

Choline Oxidase Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Choline Oxidase Native Protein (Alcaligenes gunus).
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Choline Oxidase (Alcaligenes species). Cross reactivity against Choline Oxidase from other sources is unknown.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CHOX_ALCSP
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:5000
  • ELISA 1:5000 - 1:20000
  • Immunoprecipitation 1:100
Application Notes
This product has been assayed against 1.0 ug of Choline Oxidase [Alcaligenes species] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:4,000 - 1:22,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Alcaligenes gunus of bateria.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Ion exchange chromatography

Alternate Names for Choline Oxidase Antibody

  • Choline oxydase

Background

Choline oxidase from Alcaligenes sp. catalyzes the oxidation of choline and betaine aldehyde to betaine with concomitant consumption of oxygen and production of hydrogen peroxide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Additional Choline Oxidase Products

Choline Oxidase NB600-866

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Bioinformatics

Gene Symbol CHOX_ALCSP