Choline Oxidase Antibody


Western Blot: Choline Oxidase Antibody [NB600-866] - Samples of 1 and 0.25 ug of purified Choline Oxidase per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples of purified Choline more
Western Blot: Choline Oxidase Antibody [NB600-866] - Analysis using the Biotin conjugate of NB600-866. Detection of Lane 1: Choline Oxidase (Alcaligenes species). Load: 50 ng per lane. Primary antibody: Goat more
Western Blot: Choline Oxidase Antibody [NB600-866] - Analysis using the HRP conjugate of NB600-866. Detection of antibody with Bilirubin Oxidase under reducing (R) conditions. A samples of ~1ug of purified protein more
Western Blot: Choline Oxidase Antibody [NB600-866] - Analysis using the HRP conjugate of NB600-866. Detection of Lane 1: Bilirubin Oxidase (Myrothecium verrucaria). Load: 50 ng per lane. Primary antibody: Goat more

Product Details

Reactivity BaSpecies Glossary
Applications WB, ELISA, IP

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Choline Oxidase Antibody Summary

This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Choline Oxidase Native Protein (Alcaligenes gunus).
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Choline Oxidase (Alcaligenes species). Cross reactivity against Choline Oxidase from other sources is unknown.
Ion exchange chromatography
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  • Western Blot 1:500 - 1:5000
  • ELISA 1:5000 - 1:20000
  • Immunoprecipitation 1:100
Application Notes
This product has been assayed against 1.0 ug of Choline Oxidase [Alcaligenes species] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:4,000 - 1:22,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Alcaligenes gunus of bateria.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% Sodium Azide
Ion exchange chromatography

Alternate Names for Choline Oxidase Antibody

  • Choline oxydase


Choline oxidase from Alcaligenes sp. catalyzes the oxidation of choline and betaine aldehyde to betaine with concomitant consumption of oxygen and production of hydrogen peroxide.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Additional Choline Oxidase Products

Choline Oxidase NB600-866

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Gene Symbol CHOX_ALCSP