Less than 0.1 EU/ug of Cholera Toxin Beta as determined by LAL method.
11.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP2-61449 in the following applications:
0.2 um filtered solution in 5 mM PB, pH 7.0, 75 mM NaCl.
>98%, by SDS-PAGE and HPLC
Alternate Names for Recombinant Cholera Toxin Beta Protein
Cholera enterotoxin B chain
Cholera enterotoxin beta chain
Cholera enterotoxin gamma chain
Cholera enterotoxin subunit B
Cholera toxin B protein
The holotoxin (choleragen) consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge. The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself. After binding to gangliosides GM1 in lipid rafts, through the subunit B pentamer, the holotoxin and the gangliosides are internalized. The holotoxin remains bound to GM1 until arrival in the ER. The A subunit has previously been cleaved in the intestinal lumen but the A1 and A2 chains have remained associated. In the ER, the A subunit disulfide bridge is reduced, the A1 chain is unfolded by the PDI and disassembled from the rest of the toxin. Then, the membrane-associated ER oxidase ERO1 oxidizes PDI, which releases the unfolded A1 chain. The next step is the retrotranslocation of A1 into the cytosol. This might be mediated by the protein-conducting pore SEC61. Upon arrival in the cytosol, A1 refolds and avoids proteasome degradation. In one way or another, A1 finally reaches its target and induces toxicity.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
Publications for Cholera Toxin Beta Recombinant Protein (NBP2-61449)(3)
We have publications tested in 1 confirmed species: Bacteria.
We have publications tested in 2 applications: In vitro, LIM.
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