Cholera Haemolysin Antibody (2024/251) - BSA Free


There are currently no images for Cholera Haemolysin Antibody (NB100-62460).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity BaSpecies Glossary
Applications ELISA
BSA Free
3.1 mg/ml

Order Details

Cholera Haemolysin Antibody (2024/251) - BSA Free Summary

Native organisms
NB100-62460 is specific for Vibrio cholerae haemolysin (HlyA). HlyA is an extracellular matrix damaging protein with a molecular weight of 65 kDa that exists a water-soluble monomer and an oligomeric amphipathic protein. It belongs to a class of proteins known as pore-forming toxins for their ability to punch holes in plasma membranes.
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • ELISA 1:100-1:2000

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.09% Sodium Azide
3.1 mg/ml
Protein A purified

Alternate Names for Cholera Haemolysin Antibody (2024/251) - BSA Free

  • HlyA
  • VCA0219


The holotoxin (choleragen) consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge. The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself. After binding to gangliosides GM1 in lipid rafts, through the subunit B pentamer, the holotoxin and the gangliosides are internalized. The holotoxin remains bound to GM1 until arrival in the ER. The A subunit has previously been cleaved in the intestinal lumen but the A1 and A2 chains have remained associated. In the ER, the A subunit disulfide bridge is reduced, the A1 chain is unfolded by the PDI and disassembled from the rest of the toxin. Then, the membrane-associated ER oxidase ERO1 oxidizes PDI, which releases the unfolded A1 chain. The next step is the retrotranslocation of A1 into the cytosol. This might be mediated by the protein-conducting pore SEC61. Upon arrival in the cytosol, A1 refolds and avoids proteasome degradation. In one way or another, A1 finally reaches its target and induces toxicity.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cholera Haemolysin Antibody (NB100-62460) (0)

There are no publications for Cholera Haemolysin Antibody (NB100-62460).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Cholera Haemolysin Antibody (NB100-62460) (0)

There are no reviews for Cholera Haemolysin Antibody (NB100-62460). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Cholera Haemolysin Antibody (NB100-62460) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional Cholera Haemolysin Products

Array NB100-62460

Blogs on Cholera Haemolysin

There are no specific blogs for Cholera Haemolysin, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Cholera Haemolysin Antibody (2024/251) - BSA Free and receive a gift card or discount.