Cholera Haemolysin Antibody (2024/251) - BSA Free

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Product Details

Summary
Reactivity BaSpecies Glossary
Applications ELISA
Clone
2024/251
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
3.1 mg/ml

Order Details

Cholera Haemolysin Antibody (2024/251) - BSA Free Summary

Immunogen
Native organisms
Specificity
NB100-62460 is specific for Vibrio cholerae haemolysin (HlyA). HlyA is an extracellular matrix damaging protein with a molecular weight of 65 kDa that exists a water-soluble monomer and an oligomeric amphipathic protein. It belongs to a class of proteins known as pore-forming toxins for their ability to punch holes in plasma membranes.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
3.1 mg/ml
Purity
Protein A purified

Alternate Names for Cholera Haemolysin Antibody (2024/251) - BSA Free

  • HlyA
  • VCA0219

Background

The holotoxin (choleragen) consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge. The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself. After binding to gangliosides GM1 in lipid rafts, through the subunit B pentamer, the holotoxin and the gangliosides are internalized. The holotoxin remains bound to GM1 until arrival in the ER. The A subunit has previously been cleaved in the intestinal lumen but the A1 and A2 chains have remained associated. In the ER, the A subunit disulfide bridge is reduced, the A1 chain is unfolded by the PDI and disassembled from the rest of the toxin. Then, the membrane-associated ER oxidase ERO1 oxidizes PDI, which releases the unfolded A1 chain. The next step is the retrotranslocation of A1 into the cytosol. This might be mediated by the protein-conducting pore SEC61. Upon arrival in the cytosol, A1 refolds and avoids proteasome degradation. In one way or another, A1 finally reaches its target and induces toxicity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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