Chloroquine Antibody (25G10) [Alexa Fluor® 532]

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-05105AF532
    • Availability
      Product Discontinued

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Chloroquine Antibody (25G10) [Alexa Fluor® 532] Summary

Immunogen
2-amino-5-diethylaminopentane coupled to immunogenic carrier protein
Epitope
This antibody has specificity for the non-quinoline-sidechain of chloroquine
Specificity
Has specificity for chloroquine and can be used for chloroquine quantification in human serum or plasma with a detection limit of app. 20 ng/ml.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Chemical

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Background

Chloroquine is the prototype anti malarial drug, most widely used to treat all types of malarial infections. It is also the cheapest, time tested and safe anti malarial agent. Chloroquine is a hapten of molecular weight 158.29 g/mol. The quinoline moiety of the chloroquine molecule is the same as for many other antimalarial drugs, but the side chain is unique.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Additional Chloroquine Products

Array NBP1-05105AF532

Blogs on Chloroquine.


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How to visualize autophagy by microscopy
By Christina Towers, PhD Autophagy is a recycling process that relies on the formation of a unique organelle termed an autophagosome. An elegant way to monitor autophagy is through various microscopy techniques to...  Read full blog post.


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Application Focus: I see an increase in LC3, now what?
 By Christina Towers, PhD.  Autophagy is highly conserved and tightly regulated process that all cell types use to recycle nutrients, particularly in the instance of stress1. As a result, even sm...  Read full blog post.

Measuring Autophagic Flux with LC3 protein levels: The do's and don'ts
By Christina Towers, PhD. Autophagy is a dynamic cellular recycling process that can be influenced by many different external and internal stimuli. The most commonly used assay to measure autophagy is a western blot f...  Read full blog post.

Autophagy inhibition in pediatrics: One physician-scientist’s brave decision
By Christina Towers, PhD. The current time from when a discovery is first made on the bench to when that discovery might translate into an approved therapy in cancer patients is an astounding 10-15 years. Scientis...  Read full blog post.

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