cGMP Parameter Assay Kit

Product Discontinued

cGMP Parameter Assay Kit Summary

• Background: The Parameter cGMP Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure cGMP in cell culture supernates, plasma, saliva, urine, and cell lysates.
• Additional Information: Item in the process of being discontinued
• Specificity: Measures cyclic GMP levels in various samples
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for cGMP Parameter Assay Kit

• cGMP

Background

Guanosine 3, 5-cyclic monophosphate (cGMP) is formed via the action of guanylate cyclase on GTP. cGMP is present at levels typically 10-100 fold lower than cAMP in most tissues. A variety of stimuli including acetylcholine, insulin, oxytocin, serotonin, and histamine will cause an increase in cGMP levels. Various stimulators of guanylate cyclase (e.g. vasodilators) as well as peptides that relax smooth muscle also increase cGMP concentrations.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for cGMP (KGE003)(54)

Publications using KGE003

• M Jänsch, LT Lubomirov, M Trum, T Williams, J Schmitt, K Schuh, F Qadri, LS Maier, M Bader, O Ritter Inducible over-expression of cardiac Nos1ap causes short QT syndrome in transgenic mice FEBS Open Bio, 2022-11-27;0(0):. 2022-11-27 [PMID: 36352324] (Mouse)
• N Marozkina, L Smith, Y Zhao, J Zein, JF Chmiel, J Kim, J Kiselar, MD Davis, RS Cunningham, SH Randell, B Gaston Somatic cell hemoglobin modulates nitrogen oxide metabolism in the human airway epithelium Scientific Reports, 2021-07-29;11(1):15498. 2021-07-29 [PMID: 34326365] (Human)
• A ?uczak, R Ma?ecki, M Kulus, M Madej, E Szahidewic, A Doroszko Cardiovascular Risk and Endothelial Dysfunction in Primary Sjogren Syndrome Is Related to the Disease Activity Nutrients, 2021-06-17;13(6):. 2021-06-17 [PMID: 34204342] (Human)
• TRF de Melo, BM Dulmovits, GFDS Fernandes, CM de Souza, C Lanaro, M He, Y Al Abed, MC Chung, L Blanc, FF Costa, JL Dos Santos Synthesis and pharmacological evaluation of pomalidomide derivatives useful for sickle cell disease treatment Bioorganic chemistry, 2021-06-10;114(0):105077. 2021-06-10 [PMID: 34130111] (Human)
• A de la Fuen, M Toral, A Alfayate, MJ Ruiz-Rodrí, E Bonzón-Kul, G Teixido-Tu, S Martínez-M, MJ Méndez-Oli, D López-Made, I González-V, E Garcia-Izq, S Mingo, CE Martín, L Muiño-Mosq, J De Backer, JF Nistal, A Forteza, A Evangelist, J Vázquez, MR Campanero, JM Redondo Aortic disease in Marfan syndrome is caused by overactivation of sGC-PRKG signaling by NO Nature Communications, 2021-05-11;12(1):2628. 2021-05-11 [PMID: 33976159] (Human, Mouse)
• S Sargazi, S Shahraki, O Shahraki, F Zargari, R Sheervalil, S Maghsoudi, MN Soltani Ra, R Saravani 8-Alkylmercaptocaffeine derivatives: antioxidant, molecular docking, and in-vitro cytotoxicity studies Bioorganic chemistry, 2021-04-09;111(0):104900. 2021-04-09 [PMID: 33894429] (Human)
• HT McKenna, KA O'Brien, BO Fernandez, M Minnion, A Tod, BD McNally, JA West, JL Griffin, MP Grocott, MG Mythen, M Feelisch, AJ Murray, DS Martin Divergent trajectories of cellular bioenergetics, intermediary metabolism and systemic redox status in survivors and non-survivors of critical illness Redox Biology, 2021-02-20;41(0):101907. 2021-02-20 [PMID: 33667994] (Human)
• KR Talaat, CK Porter, KM Jaep, CA Duplessis, RL Gutierrez, M Maciel, B Adjoodani, B Feijoo, S Chakrabort, J Brubaker, SA Trop, MS Riddle, SS Joseph, AL Bourgeois, MG Prouty Refinement of the CS6-expressing enterotoxigenic Escherichia coli strain B7A human challenge model: A randomized trial PLoS ONE, 2020-12-02;15(12):e0239888. 2020-12-02 [PMID: 33264302] (Human)
• J Song, T Sun, Z Tang, Y Ruan, K Liu, K Rao, R Lan, S Wang, T Wang, J Liu Exosomes derived from smooth muscle cells ameliorate diabetes-induced erectile dysfunction by inhibiting fibrosis and modulating the NO/cGMP pathway J Cell Mol Med, 2020-10-03;0(0):. 2020-10-03 [PMID: 33009701] (Rat)
• S Kim, M Park, JY Kim, T Kim, JY Hwang, KS Ha, MH Won, S Ryoo, YG Kwon, YM Kim Circulating miRNAs Associated with Dysregulated Vascular and Trophoblast Function as Target-Based Diagnostic Biomarkers for Preeclampsia Cells, 2020-08-31;9(9):. 2020-08-31 [PMID: 32878300] (Human)
• D Kolijn, Á Kovács, M Herwig, M Lódi, M Sieme, A Alhaj, P Sandner, Z Papp, PH Reusch, P Haldenwang, I Falcão-Pir, WA Linke, K Jaquet, S Van Lintho, A Mügge, C Tschöpe, N Hamdani Enhanced Cardiomyocyte Function in Hypertensive Rats With Diastolic Dysfunction and Human Heart Failure Patients After Acute Treatment With Soluble Guanylyl Cyclase (sGC) Activator Front Physiol, 2020-05-25;11(0):345. 2020-05-25 [PMID: 32523538] (Human)
• Z Wu, X Lu, S Zhang, C Zhu Sini-San Regulates the NO-cGMP-PKG Pathway in the Spinal Dorsal Horn in a Modified Rat Model of Functional Dyspepsia Evid Based Complement Alternat Med, 2020-03-31;2020(0):3575231. 2020-03-31 [PMID: 32328126] (Rat)
• IS Bae, SH Kim Expression and Secretion of an Atrial Natriuretic Peptide in Beige-Like 3T3-L1 Adipocytes Int J Mol Sci, 2019-12-05;20(24):. 2019-12-05 [PMID: 31817347] (Human)
• SM Mirczuk, AJ Lessey, AR Catterick, RM Perrett, CJ Scudder, JE Read, VJ Lipscomb, SJ Niessen, AJ Childs, CA McArdle, IM McGonnell, RC Fowkes Regulation and Function of C-Type Natriuretic Peptide (CNP) in Gonadotrope-Derived Cell Lines Cells, 2019-09-14;8(9):. 2019-09-14 [PMID: 31540096] (Human)
• X Deng, H Yasuda, T Sasaki, M Yamaya Low-Dose Carbon Monoxide Inhibits Rhinovirus Replication in Human Alveolar and Airway Epithelial Cells Tohoku J. Exp. Med., 2019-04-01;247(4):215-222. 2019-04-01 [PMID: 30971638] (Human)
• AF Cumpstey, M Minnion, BO Fernandez, M Mikus-Leli, K Mitchell, DS Martin, MPW Grocott, M Feelisch Pushing arterial-venous plasma biomarkers to new heights: A model for personalised redox metabolomics? Redox Biol, 2019-01-22;21(0):101113. 2019-01-22 [PMID: 30738322] (Human)
• M Yata, HS Kooistra, NJ Beijerink Cardiorenal and endocrine effects of synthetic canine BNP1-32 in dogs with compensated congestive heart failure caused by myxomatous mitral valve disease J. Vet. Intern. Med., 2019-01-31;0(0):. 2019-01-31 [PMID: 30703246] (Canine)
• S Kim, KS Lee, S Choi, J Kim, DK Lee, M Park, W Park, TH Kim, JY Hwang, MH Won, H Lee, S Ryoo, KS Ha, YG Kwon, YM Kim NF-?B-Responsive miRNA-31-5p elicits endothelial dysfunction associated with preeclampsia via downregulation of endothelial nitric oxide synthase J. Biol. Chem., 2018-10-02;0(0):. 2018-10-02 [PMID: 30279269] (Human)
• SS Zaidi, RD Ward, K Ramanathan, X Yu, IP Gladysheva, GL Reed Possible Enzymatic Downregulation of the Natriuretic Peptide System in Patients with Reduced Systolic Function and Heart Failure: A Pilot Study Biomed Res Int, 2018-07-26;2018(0):7279036. 2018-07-26 [PMID: 30148170] (Human)
• L Chen, C Liu, D Sun, T Wang, L Zhao, W Chen, M Yuan, J Wang, W Lu MicroRNA-133a Impairs Perfusion Recovery after Hindlimb Ischemia in Diabetic Mice Biosci. Rep., 2018-07-02;0(0):. 2018-07-02 [PMID: 29789398] (Mouse)
• M Packer, B Claggett, MP Lefkowitz, JJV McMurray, JL Rouleau, SD Solomon, MR Zile Effect of neprilysin inhibition on renal function in patients with type 2 diabetes and chronic heart failure who are receiving target doses of inhibitors of the renin-angiotensin system: a secondary analysis of the PARADIGM-HF trial Lancet Diabetes Endocrinol, 2018-04-13;0(0):. 2018-04-13 [PMID: 29661699] (Human)
• LY Zhao, J Li, XQ Huang, GH Wang, XF Lv, WF Meng, WL Chen, JY Pang, YC Lin, HS Sun, GL Wang, YM Du Xyloketal B exerts antihypertensive effect in renovascular hypertensive rats via the NO-sGC-cGMP pathway and calcium signaling Acta Pharmacol. Sin., 2018-03-29;0(0):. 2018-03-29 [PMID: 29595193] (Rat)
• J Rashid, K Nahar, S Raut, A Keshavarz, F Ahsan Fasudil and DETA NONOate, loaded in a peptide-modified liposomal carrier, slows PAH progression upon pulmonary delivery Mol. Pharm., 2018-03-26;0(0):. 2018-03-26 [PMID: 29528655] (Human)
• D Wu, Q Hu, Y Xiong, D Zhu, Y Mao, YZ Zhu Novel H2S-NO hybrid molecule (ZYZ-803) promoted synergistic effects against heart failure Redox Biol, 2017-12-14;15(0):243-252. 2017-12-14 [PMID: 29288927] (Mouse)
• S Choi, J Kim, JH Kim, DK Lee, W Park, M Park, S Kim, JY Hwang, MH Won, YK Choi, S Ryoo, KS Ha, YG Kwon, YM Kim Carbon monoxide prevents TNF-?-induced eNOS downregulation by inhibiting NF-?B-responsive miR-155-5p biogenesis Exp. Mol. Med., 2017-11-24;49(11):e403. 2017-11-24 [PMID: 29170479] (Human)
• J Jiang, Z Gan, Y Li, W Zhao, H Li, JP Zheng, Y Ke REM sleep deprivation induces endothelial dysfunction and hypertension in middle-aged rats: Roles of the eNOS/NO/cGMP pathway and supplementation with L-arginine PLoS ONE, 2017-08-15;12(8):e0182746. 2017-08-15 [PMID: 28809932] (Rat)
• L Wandrag, M Siervo, HL Riley, M Khosravi, BO Fernandez, CA Leckstrom, DS Martin, K Mitchell, DZH Levett, HE Montgomery, MG Mythen, MA Stroud, MPW Grocott, M Feelisch, Caudwell X Does hypoxia play a role in the development of sarcopenia in humans? Mechanistic insights from the Caudwell Xtreme Everest Expedition Redox Biol, 2017-05-08;13(0):60-68. 2017-05-08 [PMID: 28570949] (Human)
• R Saravani, HR Galavi, A Shahraki Inhibition of Phosphodiesterase 5 and Increasing the Level of Cyclic Guanosine 3',5' Monophosphate by Hydroalcoholic Achillea wilhelmsii C. Koch Extract in Human Breast Cancer Cell Lines MCF-7 and MDA-Mb-468 Breast Cancer (Auckl), 2017-02-23;11(0):1178223417690. 2017-02-23 [PMID: 28469435] (Human)
• N Duangnin, T Phitak, P Pothacharo, P Kongtawele In vitro and in vivo investigation of natural compounds from seed extract of Mucuna pruriens lacking l-DOPA for the treatment of erectile dysfunction Asian Pac J Trop Med, 2017-03-07;10(3):238-252. 2017-03-07 [PMID: 28442107] (Human, Rat)
• GS Dhaunsi, M Yousif, B Makki, S Akhtar, IF Benter Angiotensin-(1-7) Downregulates Diabetes-Induced cGMP Phosphodiesterase Activation in Rat Corpus Cavernosum Biomed Res Int, 2017-02-19;2017(0):5084961. 2017-02-19 [PMID: 28299329] (Rat)
• Z Liu, Y Liu, Q Xu, H Peng, Y Tang, T Yang, Z Yu, G Cheng, G Zhang, R Shi Critical role of vascular peroxidase 1 in regulating endothelial nitric oxide synthase Redox Biol, 2017-02-27;12(0):226-232. 2017-02-27 [PMID: 28264790] (Human)
• Lycopene Ameliorates Transplant Arteriosclerosis in Vascular Allograft Transplantation by Regulating the NO/cGMP Pathways and Rho-Associated Kinases Expression Oxid Med Cell Longev, 2016-12-05;2016(0):3128280. 2016-12-05 [PMID: 28050227] (Rat)
• Unbalanced Oxidant-Antioxidant Status: A Potential Therapeutic Target for Coronary Chronic Total Occlusion in Very Old Patients Oxid Med Cell Longev, 2016-12-01;2016(0):4910829. 2016-12-01 [PMID: 28044093] (Rat)
• Hoi I Cheong Alternative hematological and vascular adaptive responses to high-altitude hypoxia in East African Highlanders Am. J. Physiol. Lung Cell Mol. Physiol, 2016-12-15;0(0):ajplung.00451. 2016-12-15 [PMID: 27979860] (Human)
• S-palmitoylation of a Novel Site in the ?2-adrenergic Receptor Associated with a Novel Intracellular Itinerary J Biol Chem, 2016-08-01;0(0):. 2016-08-01 [PMID: 27481942] (Human)
• CP Hsu, JF Zhao, SJ Lin, SK Shyue, BC Guo, TM Lu, TS Lee Asymmetric Dimethylarginine Limits the Efficacy of Simvastatin Activating Endothelial Nitric Oxide Synthase J Am Heart Assoc, 2016-04-18;5(4):. 2016-04-18 [PMID: 27091343] (Human)
• D Chen, KQ Zhang, B Li, DQ Sun, H Zhang, Q Fu Epigallocatechin-3-gallate ameliorates erectile function in aged rats via regulation of PRMT1/DDAH/ADMA/NOS metabolism pathway Asian J Androl, 2017-05-01;0(0):. 2017-05-01 [PMID: 27080477] (Rat)
• AM van der Gr, ND Paauw, TJ Toering, M Feelisch, MM Faas, TR Sutton, MM Minnion, JD Lefrandt, SA Scherjon, A Franx, G Navis, AT Lely Impaired sodium dependent adaptation of arterial stiffness in formerly preeclamptic women: The RETAP - vascular study Am J Physiol Heart Circ Physiol, 2016-04-08;0(0):ajpheart.0001. 2016-04-08 [PMID: 27059075] (Human)
• Zhang Q, Wang G, Yuan W, Wu J, Wang M, Li C The effects of phosphodiesterase-5 inhibitor sildenafil against post-resuscitation myocardial and intestinal microcirculatory dysfunction by attenuating apoptosis and regulating microRNAs expression: essential role of nitric oxide syntheses signaling. J Transl Med, 2015-06-04;13(0):177. 2015-06-04 [PMID: 26040988] (Porcine)
• Read L, Hahn R, Thompson C, Bauer D, Norton E, Clements J Simultaneous exposure to Escherichia coli heat-labile and heat-stable enterotoxins increases fluid secretion and alters cyclic nucleotide and cytokine production by intestinal epithelial cells. Infect Immun, 2014-10-06;82(12):5308-16. 2014-10-06 [PMID: 25287923] (Human)
• Maruhashi , Tatsuya, Noma , Kensuke, Iwamoto , Yumiko, Iwamoto , Akimichi, Oda , Nozomu, Kajikawa , Masato, Matsumoto , Takeshi, Hidaka , Takayuki, Kihara , Yasuki, Chayama , Kazuaki, Nakashima , Ayumu, Goto , Chikara, Liao , James K, Higashi , Yukihito Critical role of exogenous nitric oxide in ROCK activity in vascular smooth muscle cells. PLoS ONE, 2014-10-03;9(10):e109017. 2014-10-03 [PMID: 25280018] (Human, Mouse)
• Pan , Dongning, Mao , Chunxiao, Quattrochi , Brian, Friedline , Randall, Zhu , Lihua J, Jung , Dae Youn, Kim , Jason K, Lewis , Brian, Wang , Yong-Xu MicroRNA-378 controls classical brown fat expansion to counteract obesity. Nat Commun, 2014-08-22;5(0):4725. 2014-08-22 [PMID: 25145289] (Mouse)
• Arab H, Al-Shorbagy M, Abdallah D, Nassar N Telmisartan attenuates colon inflammation, oxidative perturbations and apoptosis in a rat model of experimental inflammatory bowel disease. PLoS ONE, 2014-05-15;9(5):e97193. 2014-05-15 [PMID: 24831514] (Human)
• Myojo , Masahiro, Nagata , Daisuke, Fujita , Daishi, Kiyosue , Arihiro, Takahashi , Masao, Satonaka , Hiroshi, Morishita , Yoshiyuk, Akimoto , Tetsu, Nagai , Ryozo, Komuro , Issei, Hirata , Yasunobu Telmisartan activates endothelial nitric oxide synthase via Ser1177 phosphorylation in vascular endothelial cells. PLoS ONE, 2014-05-14;9(5):e96948. 2014-05-14 [PMID: 24827148] (Human)
• Wang Q, Zheng X, Cheng Y, Zhang Y, Wen H, Tao Z, Li H, Hao Y, Gao Y, Yang L, Smith F, Huang C, Jin S Resolvin D1 stimulates alveolar fluid clearance through alveolar epithelial sodium channel, Na,K-ATPase via ALX/cAMP/PI3K pathway in lipopolysaccharide-induced acute lung injury. J Immunol, 2014-03-19;192(8):3765-77. 2014-03-19 [PMID: 24646745] (Rat)
• Dada , Jessica, Pinder , Andrew G, Lang , Derek, James , Philip E Oxygen mediates vascular smooth muscle relaxation in hypoxia. PLoS ONE, 2013-02-22;8(2):e57162. 2013-02-22 [PMID: 23451175]
• Ibebuogu UN, Gladysheva IP, Houng AK Decompensated heart failure is associated with reduced corin levels and decreased cleavage of pro-atrial natriuretic peptide. Circ Heart Fail, 2011-01-07;4(2):114-20. 2011-01-07 [PMID: 21216831] (Human)
• Little JA, Hauser KP, Martyr SE, Harris A, Maric I, Morris CR, Suh JH, Taylor J, Castro O, Machado R, Kato G, Gladwin MT Hematologic, biochemical, and cardiopulmonary effects of L-arginine supplementation or phosphodiesterase 5 inhibition in patients with sickle cell disease who are on hydroxyurea therapy. Eur. J. Haematol., 2008-02-10;82(4):315-21. 2008-02-10 [PMID: 19215288] (Human)
• Reeve VE, Tyrrell RM, Allanson M, Domanski D, Blyth L The role of interleukin-6 in UVA protection against UVB-induced immunosuppression. J. Invest. Dermatol., 2008-12-25;129(6):1539-46. 2008-12-25 [PMID: 19110542] (Mouse)
• Lema G, Urzua J, Jalil R, Canessa R, Vogel A, Moran S, Fajuri A, Carvajal C, Aeschlimann N, Jaque MP Decreased nitric oxide products in the urine of patients undergoing cardiac surgery. J. Cardiothorac. Vasc. Anesth., 2008-11-20;23(2):188-94. 2008-11-20 [PMID: 19026569] (Human)
• Tradtrantip L, Yangthara B, Padmawar P, Morrison C, Verkman AS Thiophenecarboxylate suppressor of cyclic nucleotides discovered in a small-molecule screen blocks toxin-induced intestinal fluid secretion. Mol. Pharmacol., 2008-09-29;75(1):134-42. 2008-09-29 [PMID: 18824527] (Hamster)
• Haramis G, Zhou Z, Pyriochou A, Koutsilieris M, Roussos C, Papapetropoulos A cGMP-independent anti-tumour actions of the inhibitor of soluble guanylyl cyclase, ODQ, in prostate cancer cell lines. Br. J. Pharmacol., 2008-08-11;155(6):804-13. 2008-08-11 [PMID: 18695639] (Human)
• Jangi SM, Ruiz-Larrea MB, Nicolau-Galmes F, Andollo N, Arroyo-Berdugo Y, Ortega-Martinez I, Diaz-Perez JL, Boyano MD Terfenadine-induced apoptosis in human melanoma cells is mediated through Ca2+ homeostasis modulation and tyrosine kinase activity, independently of H1 histamine receptors. Carcinogenesis, 2008-01-03;29(3):500-9. 2008-01-03 [PMID: 18174239] (Human)
• Pankow K, Wang Y, Gembardt F, Krause E, Sun X, Krause G, Schultheiss HP, Siems WE, Walther T Successive action of meprin A and neprilysin catabolizes B-type natriuretic peptide. Circ. Res., 2007-09-06;101(9):875-82. 2007-09-06 [PMID: 17823376] (Human, Mouse)