Cell-Mediated Cytotoxicity Assay Kit (Fluorometric)


There are currently no images for Cell-Mediated Cytotoxicity Kit (NBP2-54852).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications Flow
Suitable Sample Type
Adherent and suspension cells

Order Details

Cell-Mediated Cytotoxicity Assay Kit (Fluorometric) Summary

Direct method to measure cell-mediated cytotoxicity. Does not require cell lysis and provides multi-parametric analysis.
  • Detection method: Fluorescence (FL1 channel and FL3 channel)
  • Applications: Measurement of cell cytotoxicity in response to drug or toxin treatment. Quantification of the cytotoxic effect of immune effector cells on target cells. Assessment of physiological mediators and antibodies that affect cell cytotoxicity.
Kit Type
Assay Kit (Fluorometric)


  • Flow Cytometry
Application Notes
Staining treated cells with CFSE alone and 7-AAD alone to choose the proper instrument gating set up. Keep unstained control cells (i.e. without CFSE or 7-AAD staining) suspended in Cytotoxicity Assay Buffer for both treated and untreated samples to set up the flow cytometer. CFSE cell staining is measured in the FL1 channel and 7-AAD cell staining is measured in the FL3 channel. To ensure that only proper target cells are gated, use a side scatter versus FL-1 plot.

Packaging, Storage & Formulations

Store at -20C.

Kit Components

  1. 7-AAD Staining Solution
  2. CFSE Staining Solution
  3. Cytotoxicity Assay Buffer


Cell-mediated cytotoxicity is an important phenomenon characterized by cytolysis of a compromised cell in the body by immune system. Activation of immune system leads to removal of target cells infected by pathogens or transformed cells/cancer cells. This process is mediated by antibody-dependent cell-mediated cytotoxicity (ADCC), complement-mediated cytotoxicity, or lymphocyte-mediated cytotoxicity. This Cell-Mediated Cytotoxicity Assay Kit contains carboxyfluorosuccinimide ester (CFSE), a green fluorescent probe that labels live target cells and 7-aminoactinomycin D (7-AAD), a red fluorescent probe that labels late apoptotic and necrotic target cells killed in the cytotoxicity assay. This assay does not require cell lysis and provides a direct measurement of cytotoxicity instead of indirect indicators such as release of ATP or lactate dehydrogenase activity.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Cell-Mediated Cytotoxicity Kit (NBP2-54852) (0)

There are no publications for Cell-Mediated Cytotoxicity Kit (NBP2-54852).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Cell-Mediated Cytotoxicity Kit (NBP2-54852) (0)

There are no reviews for Cell-Mediated Cytotoxicity Kit (NBP2-54852). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Cell-Mediated Cytotoxicity Kit (NBP2-54852) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Cell-Mediated Cytotoxicity Products

Blogs on Cell-Mediated Cytotoxicity

There are no specific blogs for Cell-Mediated Cytotoxicity, but you can read our latest blog posts.

Contact Information

Product PDFs

Review this Product

Be the first to review our Cell-Mediated Cytotoxicity Assay Kit (Fluorometric) and receive a gift card or discount.