CD9 Immunobeads for Exosome Isolation (Mouse)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IA
Suitable Sample Type
Biofluids (plasma serum)

Order Details

CD9 Immunobeads for Exosome Isolation (Mouse) Summary

Description
CD9 Immunocapture Beads are 0.4 um latex beads covalently coupled with antibodies against extracellular vesicle (EV) surface antigen CD9. Samples containing EVs are incubated with Immunocapture Beads for rapid and specific isolation of EVs expressing CD9.

Using the supplied Elution Buffer, EVs can be eluted from Immunocapture beads and used in downstream analyses such as protein profiling and nucleic acid extraction.

Immunobeads can be re-used after incubation with Bead Regeneration Buffer.
Sample Volume
0.5 - 1 mL

Applications/Dilutions

Dilutions
  • Immunoassay

Packaging, Storage & Formulations

Storage
Store at 4C.

Kit Components

Components
  1. Bead Regeneration Buffer
  2. CD9 Immunocapture Beads
  3. Exosome Elution Buffer

Notes

Shelf life of 8 months.

Alternate Names for CD9 Immunobeads for Exosome Isolation (Mouse)

  • EV isolation
  • exosome isolation
  • extracellular vesicle isolation

Background

Immunocapture for isolation of exosomes relies on the antigen-antibody interaction. Antibodies directed at target of interest (eg CD9, CD63) are coated either in a 96-well plate or conjugated to a bead. The sample is applied directly into coated well, or into sample with coated beads, and EVs are allowed to bind. In a final step, EVs can be eluted from the antibody and used in downstream functional and phenotypic analysis. This method of isolation is highly specific, resulting in extremely pure EV populations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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