Carbo-Free Blocking Solution

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Product Image: Carbo-Free Blocking Solution [SP-5040-125-NB]

Product Details

Summary
Applications ELISA, ICC/IF, IHC, WB, ELISA, NULL

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Carbo-Free Blocking Solution Summary

Description
The Carbo-Free Blocking Solution is a protein-based agent used as a general blocking or diluent solution. It is ideal for applications using lectins in which glycoprotein contamination could generate background staining or false positive results.

Features:

  • Essentially free of glycoproteins, unlike other common protein-containing blocking agents like serum, nonfat dry milk, or casein
  • Supplied as 125 mL of 10X concentrate
  • Applications/Dilutions

    Dilutions
    • ELISA
    • Enzyme-linked Immunosorbent Spot
    • Immunoblotting
    • Immunocytochemistry/ Immunofluorescence
    • Immunohistochemistry
    • Product Image
    Application Notes
    Prior to use, 10X Carbo-Free Blocking Solution should be diluted to 1X with distilled or deionized water. For prolonged storage of the 1X solution, sodium azide should be added to give a final concentration of 0.08% (w/v) and the diluted solution refrigerated. Carbo-Free Blocking Solution does not contain detergent. In some applications, blocking may be enhanced by addition of Tween 20 to a final concentration of 0.05% to 0.1%, in the working solution..

    Packaging, Storage & Formulations

    Storage
    Store at 4C.

    Notes

    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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    Product General Protocols

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    Video Protocols

    ICC/IF Video Protocol

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