Canine IgG, Fab Native Protein

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SDS-Page: Canine IgG, Fab Native Protein [NBP1-97024] - SDS-Page of Dog IgG Fab Fragment. Lane 1: Dog Fab - Reduced. Lane 2: Dog Fab - Non-reduced. Load: 1.0 ug per lane. Predicted/Observed size: 25 kDa - Reduced, 50 ...read more

Product Details

Summary
Reactivity CaSpecies Glossary
Applications WB, ELISA, IP, PAGE
Concentration
LYOPH

Order Details

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Canine IgG, Fab Native Protein Summary

Description
Native Canine IgG, Fab protein.

Source: Canine Serum

Preparation
Method
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog IgG, anti-Dog IgG F(ab')2 and anti-Dog Serum. No reaction was observed against anti-Dog IgG F(c) or anti- Papain.
Protein/Peptide Type
Native Protein
Purity
Immunoelectrophoresis (IEP)

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
Lyophilized from 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunoelectrophoresis (IEP)
Reconstitution Instructions
Reconstitute with 1.0 ml deionized water.

Notes

For extended storage, aliquot reconstituted liquid and freeze at -20 C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 C as an undiluted liquid. Dilute only prior to immediate use.

Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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