Calcein Blue, AM Solution

Product Discontinued

Calcein Blue, AM Solution Summary

• Description: Calcein Blue AM is a viability dye that can be used in permeabilized cells. Upon uptake by a live cell, Calcein Blue AM is cleaved by intracellular esterases resulting in fluorescence. Dead cells will not have sufficient esterase activity and will not fluoresce. In this way, dead cells may be identified by their low Calcein Blue AM signal. Calcein Blue AM is efficiently excited by a UV laser and emits at 450nm.

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: Calcein Blue AM should be used at as low a final working concentrations as possible to minimize potential artifacts (usually between 1 and 10 uM). Generally, 15 minutes to 1 hour is sufficient for cellular uptake and processing of the dyes.

Packaging, Storage & Formulations

• Storage: Store at -20C in the dark. Avoid freeze-thaw cycles.
• Buffer: DMSO
• Preservative: No Preservative
• Concentration: Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Alternate Names for Calcein Blue, AM Solution

• Calcein Blue
• Calcein Blue, AM
• Calcein, AM

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.