Caco-2 Whole Cell Lysate

• Reactivity: Hu
• Applications: WB
• Concentration: 2 mg/ml

Caco-2 Whole Cell Lysate Summary

• Description: GROWTH MEDIA: ATCC medium (Minimum essential medium (Eagle) with 2mM L-glutamine, 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, and 20% FBS.)
• Specificity: Caco-2 (Human Colorectal Adenocarcinoma)

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -70C long term. Avoid freeze-thaw cycles.
• Buffer: SDS sample buffer containing 50 mM DTT.
• Concentration: 2 mg/ml

Lysate Details for Array

• Type: Cell
• Tissue: Digestive/Waste
• Tissue Condition: Adenocarcinoma
• Subcellular Fraction: Whole

Background

Caco-2 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The Caco-2 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.