Human C5b-9 ELISA Kit (Colorimetric)

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ELISA: Human C5b-9 ELISA Kit (Colorimetric) [NBP2-66708] - Samples were spiked with high concentrations of Human C5b-9 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Human C5b-9 ELISA Kit (Colorimetric) [NBP2-66708] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
31.25 - 2000 ng/mL
Sensitivity
18.75 ng/mL

Order Details

Human C5b-9 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human sC5b9 (soluble terminal complement complex C5b-9) in samples. No significant cross-reactivity or interference between Human sC5b9 and analogues was observed.
Standard Curve Range
31.25 - 2000 ng/mL
Sensitivity
18.75 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 5.07
Intra-Assay
%CV < 4.96
Spike Recovery
87 - 101%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publication using NBP2-66708.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human C5b-9 ELISA Kit (Colorimetric)

  • MAC
  • Membrane attack complex
  • sC5-b9
  • sC5b-9
  • TCC
  • Terminal complement complex

Background

C5b-9 is also known as the terminal complement complex (TCC). The TCC consists of C5b, C6, C7, C8 and C9 and forms the membrane attack complex (MAC) as well as the non-lytic fluid-phase SC5b-9 complex (with protein S). The MAC forms channels in target cell membranes leading to cell lysis by osmotic leakage. The complexes contain neoantigens that are absent from the individual native components from which they are formed and this antibody is directed against a neoepitope exposed on C9 when incorporated into the TCC (1,2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for C5b-9 ELISA Kit (NBP2-66708)(1)

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Product General Protocols

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FAQs for C5b-9 ELISA Kit (NBP2-66708). (Showing 1 - 1 of 1 FAQ).

  1. I would like to use ELISA kit NBP2-66708 to find the C5b-9 concentration in human plasma. How do you make the C5b-9 reference standard?
    • We have tested this kit with human plasma samples and the reference value is about 100~500ng/mL. The reference standard applied for this item is purified natural human SC5b9. Unfortunately we cannot provide its commercial resource because of proprietary reasons.

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