Human C5b-9 ELISA Kit (Colorimetric)

ELISA: Human C5b-9 ELISA Kit (Colorimetric) [NBP2-66708] - Samples were spiked with high concentrations of Human C5b-9 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human C5b-9 ELISA Kit (Colorimetric) [NBP2-66708] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell Supernatant, Tissue Homogenate
• Standard Curve Range: 31.25 - 2000 ng/mL
• Sensitivity: 18.75 ng/mL

Human C5b-9 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human sC5b9 (soluble terminal complement complex C5b-9) in samples. No significant cross-reactivity or interference between Human sC5b9 and analogues was observed.
• Standard Curve Range: 31.25 - 2000 ng/mL
• Sensitivity: 18.75 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 5.07
• Intra-Assay: %CV < 4.96
• Spike Recovery: 87 - 101%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human C5b-9 ELISA Kit (Colorimetric)

• MAC
• Membrane attack complex
• sC5-b9
• sC5b-9
• TCC
• Terminal complement complex

Background

C5b-9 is also known as the terminal complement complex (TCC). The TCC consists of C5b, C6, C7, C8 and C9 and forms the membrane attack complex (MAC) as well as the non-lytic fluid-phase SC5b-9 complex (with protein S). The MAC forms channels in target cell membranes leading to cell lysis by osmotic leakage. The complexes contain neoantigens that are absent from the individual native components from which they are formed and this antibody is directed against a neoepitope exposed on C9 when incorporated into the TCC (1,2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for C5b-9 ELISA Kit (NBP2-66708)(2)

Publications using NBP2-66708

• Jiang S, Qi J, Pan T et al. KLF4 overexpression protects against complement-mediated endothelial injury in transplant-associated thrombotic microangiopathy. Haematologica 2025-08-07 [PMID: 40820810]
• María Luisa Rodríguez de la Concepción, Erola Ainsua-Enrich, Esteban Reynaga, Carlos Ávila-Nieto, Jose Ramón Santos, Silvia Roure, Lourdes Mateu, Roger Paredes, Jordi Puig, Juan Manuel Jimenez, Nuria Izquierdo-Useros, Bonaventura Clotet, María Luisa Pedro-Botet, Jorge Carrillo High-dose intravenous immunoglobulins might modulate inflammation in COVID-19 patients Life Science Alliance 2021-07-28 [PMID: 34321327]

FAQs for C5b-9 ELISA Kit (NBP2-66708). (Showing 1 - 2 of 2 FAQs).

1. I would like to use ELISA kit NBP2-66708 to find the C5b-9 concentration in human plasma. How do you make the C5b-9 reference standard?
   • We have tested this kit with human plasma samples and the reference value is about 100~500ng/mL. The reference standard applied for this item is purified natural human SC5b9. Unfortunately we cannot provide its commercial resource because of proprietary reasons.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.