Human C5b-9 ELISA Kit (Colorimetric) Summary
Specificity |
This kit recognizes Human sC5b9 (soluble terminal complement complex C5b-9) in samples. No significant cross-reactivity or interference between Human sC5b9 and analogues was observed. |
Standard Curve Range |
31.25 - 2000 ng/mL |
Sensitivity |
18.75 ng/mL |
Assay Type |
Sandwich-ELISA |
Inter-Assay |
%CV < 5.07 |
Intra-Assay |
%CV < 4.96 |
Spike Recovery |
87 - 101% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
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- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
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Alternate Names for Human C5b-9 ELISA Kit (Colorimetric)
Background
C5b-9 is also known as the terminal complement complex (TCC). The TCC consists of C5b, C6, C7, C8 and C9 and forms the membrane attack complex (MAC) as well as the non-lytic fluid-phase SC5b-9 complex (with protein S). The MAC forms channels in target cell membranes leading to cell lysis by osmotic leakage. The complexes contain neoantigens that are absent from the individual native components from which they are formed and this antibody is directed against a neoepitope exposed on C9 when incorporated into the TCC (1,2).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
Publications for C5b-9 ELISA Kit (NBP2-66708)(1)
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1 of 1.
Publication using NBP2-66708 |
Applications |
Species |
María Luisa Rodríguez de la Concepción, Erola Ainsua-Enrich, Esteban Reynaga, Carlos Ávila-Nieto, Jose Ramón Santos, Silvia Roure, Lourdes Mateu, Roger Paredes, Jordi Puig, Juan Manuel Jimenez, Nuria Izquierdo-Useros, Bonaventura Clotet, María Luisa Pedro-Botet, Jorge Carrillo High-dose intravenous immunoglobulins might modulate inflammation in COVID-19 patients Life Science Alliance 2021-07-28 [PMID: 34321327] |
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for C5b-9 ELISA Kit (NBP2-66708). (Showing 1 - 1 of 1 FAQ).
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I would like to use ELISA kit NBP2-66708 to find the C5b-9 concentration in human plasma. How do you make the C5b-9 reference standard?
- We have tested this kit with human plasma samples and the reference value is about 100~500ng/mL. The reference standard applied for this item is purified natural human SC5b9. Unfortunately we cannot provide its commercial resource because of proprietary reasons.
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