C5b-9 Antibody (aE11) [Janelia Fluor® 635]

Product Discontinued

C5b-9 Antibody (aE11) [Janelia Fluor® 635] Summary

• Immunogen: Purified C5b-9
• Epitope: Binds to a neoepitope exposed on C9.
• Specificity: Binds both membrane-bound MAC (active) and fluid-phase SC5b-9 complexes (inactive).
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Use in Bacteria reported in scientific literature (PMID:32900809).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for C5b-9 Antibody (aE11) [Janelia Fluor® 635]

• MAC
• Membrane attack complex
• sC5-b9
• sC5b-9
• TCC
• Terminal complement complex

Background

C5b-9 is also known as the terminal complement complex (TCC). The TCC consists of C5b, C6, C7, C8 and C9 and forms the membrane attack complex (MAC) as well as the non-lytic fluid-phase SC5b-9 complex (with protein S). The MAC forms channels in target cell membranes leading to cell lysis by osmotic leakage. The complexes contain neoantigens that are absent from the individual native components from which they are formed and this antibody is directed against a neoepitope exposed on C9 when incorporated into the TCC (1,2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.