C3orf80 Antibody

Immunohistochemistry-Paraffin: C3orf80 Antibody [NBP2-48872] - Staining of human fallopian tube shows strong positivity in cilia of glandular cells.

Product Discontinued

C3orf80 Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: PDDDDDSPALLRDEAAAGSQDSLLDSGG
• Predicted Species: Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: C3orf80
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for C3orf80 Antibody

• Chromosome 3 Open Reading Frame 80

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for C3orf80 Antibody (NBP2-48872) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 11/12/2021

Summary

• Application: Western Blot
• Sample Tested: Bone Marrrow Derived Macrophages
• Species: Mouse
• Lot: R4432

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.*** Mouse bone-marrow-derived macrophages were stimulated with 100 ng/ml for 0.5, 1, 2, 3, 4, 6, 8, 24 h or left unstimulated (as control, label as 0 h) in DMEM+10% FBS, and lysed in lysis buffer (20 mM HEPES, pH7.4, 50 mM beta-glycerol phosphate, 2 mM EGTA, 1 mM DTT, 10 mM NaF, 1 mM Sodium Orthovanadate, 1% Triton-100 , 10% Glycerol, and various protease and phosphatase inhibitors). Lysates were resoled on 10% NuPAGE gel under denature condition. After transfering to nitrocellulose membrane, the blot was blocked in TBST containing 5% (w/v) Blotting Grade Blocker Non Fat Dry Milk (Bio-Rad) for 1.5 h at room temperature. After blocking, the membrane was incubated with the primary antibody diluted 1:500 in TBST containing 5% BSA and 0.02% sodium azide for 1.5 h at room temperature. After 3 washes with TBST (5', 10' and 15'), the blot was incubated with HRP-conjugated goat anti-rabbit secondary antibody (1:15,000) for 1 h at room temperature. After washing 3 times with TBST, the image was developed using ECL.

Bioinformatics

• Gene Symbol: C3orf80