C2C12 Whole Cell Lysate


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Product Details

Reactivity MuSpecies Glossary
Applications WB
2 mg/ml

Order Details

C2C12 Whole Cell Lysate Summary

GROWTH MEDIA: DMEM & 10% FBS (fetal bovine serum)
C2C12 (muscle; myoblast)


  • Western Blot

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -70C long term. Avoid freeze-thaw cycles.
SDS sample buffer containing 50 mM DTT.
2 mg/ml

Lysate Details for Array

Stem Cell
Tissue Condition
Subcellular Fraction


C2C12 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The C2C12 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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WB Video Protocol

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Array NBP2-10268

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