C1orf50 Antibody (OTI1C6)

Western Blot: C1orf50 Antibody (1C6) [NBP2-00778] Analysis of extracts (35ug) from 9 different cell lines by using anti-C1orf50 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: C1orf50 Antibody (1C6) [NBP2-00778] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY C1orf50.

Immunohistochemistry-Paraffin: C1orf50 Antibody (OTI1C6) [NBP2-00778] - C1orf50 Antibody (1C6) [NBP2-00778] - Staining of paraffin-embedded Human lymphoma tissue using anti-C1orf50 mouse monoclonal antibody.

Flow Cytometry: C1orf50 Antibody (1C6) [NBP2-00778] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-C1orf50 antibody, and then analyzed by flow cytometry.

Western Blot: C1orf50 Antibody (1C6) [NBP2-00778] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C1orf50 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C1orf50.

Immunohistochemistry-Paraffin: C1orf50 Antibody (1C6) [NBP2-00778] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-C1orf50 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: C1orf50 Antibody (1C6) [NBP2-00778] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-C1orf50 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: C1orf50 Antibody (1C6) [NBP2-00778] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-C1orf50 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: C1orf50 Antibody (1C6) [NBP2-00778] - Staining of paraffin-embedded Human breast tissue using anti-C1orf50 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: C1orf50 Antibody (1C6) [NBP2-00778] - Staining of paraffin-embedded Human Kidney tissue using anti-C1orf50 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: C1orf50 Antibody (1C6) [NBP2-00778] - Staining of paraffin-embedded Human Ovary tissue using anti-C1orf50 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Ca, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI1C6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.21 mg/ml

C1orf50 Antibody (OTI1C6) Summary

• Description: Novus Biologicals Mouse C1orf50 Antibody (OTI1C6) (NBP2-00778) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human (NP_077002) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: C1orf50
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-1000
• Theoretical MW: 21.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.21 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for C1orf50 Antibody (OTI1C6)

• chromosome 1 open reading frame 50
• hypothetical protein LOC79078
• MGC955

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C1orf50
• Entrez:
  • 79078(Human)