Human Breast Tissue MicroArray (Cancer/Normal Adjacent)

Product Discontinued

Human Breast Tissue MicroArray (Cancer/Normal Adjacent) Summary

• Description: Microarray Panel:
  Breast cancer-normal
  No. of samples: 60
  No. of patients: 15
  Core diameter: 2.0 mm
  Section thickness: 4 micrometer
  Please see manual for tissue information and location.
  
  All tissues are fixed in 10% neutral buffered formalin for 12 to 24 hours, dehydrated with gradient ethanol, cleared with xylene, and embedded in paraffin. Then each slide is tested for immunohistochemistry on multiple antibodies including the p53 protein. Quality control is ensured as every tissue block is collected and arranged by certified pathologists, who used an identical process for their own research.
  
  About 100,000 cells are included in each sample with 2 mm core diameter. Some slides may have less than 60 cores due to sample loss when obtaining multiple sections. Maximum number of missing cores in a slide is less than 10%.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Slide Format: Tissue Microarrays
  Cores: 60
  Layout: 6 x 10
  Diameter: 4.0
  Thickness: Tissue Microarrays

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: These slides are paraffin coated to prevent sample oxidization, it is recommended that slides are first de-paraffinized by baking at 62 degrees C for 1 hour in a vertical orientation prior to performing antigen retrieval procedures.

Details for Array

• Type: Tissue
• Tissue Condition: Cancer/Normal
• Position: Adjacent

Notes

Patient survival data is now available for this slide. Data available by request. Please contact us to receive sample slide data.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Tissue Micro Arrays are guaranteed for 1 year from date of receipt.

Publications for Breast Tissue Micro Array (NBP2-30172)(8)

Publications using NBP2-30172

• Hashemi-Sadraei N, Muller-Greven GM, Abdul-Karim FW et al. Expression of LC3B and FIP200/Atg17 in brain metastases of breast cancer J. Neurooncol. 2018-08-09 [PMID: 30094720] (IF/IHC, Human)
• Schmitt DC, Madeira da Silva L, Zhang W et al. ErbB2-intronic microRNA-4728: a novel tumor suppressor and antagonist of oncogenic MAPK signaling. Cell Death Dis. 2015-05-08 [PMID: 25950472] (IHC-P, ISH)
• Privette Vinnedge LM, Benight NM, Wagh PK et al. The DEK oncogene promotes cellular proliferation through paracrine Wnt signaling in Ron receptor-positive breast cancers. Oncogene. 2014-06-23 [PMID: 24954505] (IHC-P, Human)
  
  Details:
  Figs 6D, S5A
• Liu P, Begley M, Michowski W et al. Cell-cycle-regulated activation of Akt kinase by phosphorylation at its carboxyl terminus. Nature 2014-03-09 [PMID: 24670654] (IHC-P, Human)
  
  Details:
  Fig S8, slides from serially cut array tissue slides were stained with cyclin A2 or Akt-pS477/pT479 antibodies.
• Liu NN, Xi Y, Callaghan MU et al. SMAD4 is a potential prognostic marker in human breast carcinomas. Tumour Biol. 2014-01-01 [PMID: 23975369]
  
  Details:
  IHC (P): Fig S1, Tables S1 & S2. NBP2-30212/IMH-364 lot CBA3 was used in this study. Overall SMAD4 expression was significantly lower in breast cancer than in normal adjacent breast tissue.
• Liao Y, Wei Y, Zhou X et al. Peptidyl-prolyl cis/trans isomerase Pin1 is critical for the regulation of PKB/Akt stability and activation phosphorylation. Oncogene. 2009-07-02 [PMID: 19448664]
• Li X, Jia Z, Shen Y et al. Coordinate suppression of Sdpr and Fhl1 expression in tumors of the breast, kidney, and prostate. Cancer Sci. 2008-07-01 [PMID: 18422756] (IHC-P)
  
  Details:
  IHC (paraffin), Fig 1 and Table 1: 1. IMH-303 (Prostate cancer) 2. IMH-313 (Kidney, cancer-normal adjacent tissues) 3. IMH-371 [Breast: cancer-normal adjacent (60 samples)].
• Smuczek B. Peptideo C16, derivado da laminina, regulando a expressao de potenciais biomarcadorers do câncer de mama / Peptide C16 derived from laminin, regulate the expression of potential biomarkers of breast Thesis. 2014-01-01 (IHC-P)