Breast Matched Tumor and Normal Tissue Lysate

• Reactivity: Hu

Breast Matched Tumor and Normal Tissue Lysate Summary

• Description: 1 x Human breast tumor tissue lysate (1 mg/ml, 100 ug/vial)
  1 x Human breast normal tissue lysate (matched) (1 mg/ml, 100 ug/vial)
  

  Matched Tumor & Normal Tissue Lysate Set

  Diagnosis: Invasive ductal carcinoma
  
  Sex: Female
  
  Age: 51
  
  Grade: 1
  
  Stage: IIB, T₂N₁M₀
  
  Tumor Pathology Data
  
  Location: Right breast
  
  Gross findings: Tumor size 4 x 3 x 3 cm. Cut section hard and white/gray. Ipsilateral axillary lymph nodes examined: 23
  
  Microscopic Findings
  
  Cancer in surgical margin of section: Negative
  Multifocal carcinoma: Negative
  Pectoral muscular/facia invasion: Negative
  Chest wall invasion: Negative
  Breast skin dermal invasion: Negative
  Dermal Lymphatic channel invasion: Negative
  Skin invasion with ulceration: Negative
  Dermal lymphatic channel invasion: Negative
  Lymphatic and/or blood vessel invasion: Negative
  Tumor necrosis: Negative
  Number of nodes positive: 5/23
  Tubule formation: 2
  Nuclear pleomorphism: 2
  Mitotic activity: 1
  Lymphocytic response: Positive
  
  Preparation Method
  
  Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify.
  Extraction 1: PBS, pH 7.4; 1 ug/ml Aprotinin; 1 mM NaF
  Modified RIPA Buffer: 1 mM EDTA; 1 ug/ml Pepstatin-A; 0.1% SDS; 0.25% Na deoxycholate; 1 ug/ml Leupeptin; 1 mM PMSF; 1 mM Na₃VO₄

Applications/Dilutions

• Application Notes: These lysates have not been subjected to denaturing or reducing conditions. This allows the tissue or cell lysate to be used in a variety of applications; to study protein-protein interaction, ligand binding, ELISA, immunoprecipitation, 1D and 2D gel electrophoresis, and Western blotting for the detection of specific protein targets.
  For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required.
  Buffer requirements for performing protein-protein interaction and ligand binding studies can vary significantly from RIPA buffer and may require modifications. In most cases, tissue lysates in RIPA buffer can be used, directly in standard ELISA and immunoprecipitation assays.
  These lysates are proteomic discovery tools. Researchers should validate and optimize for individual use.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.

Lysate Details for Array

• Type: Matched Tumor and Normal Tissue
• Life Stage: Adult
• Tissue: Breast

Notes

The vial is provided with a 10% overfill. Maximum recovery can be obtained by centrifuging the vial briefly to collect any solution on the cap and tube sides.

This material has tested negative for HbsAg, HIV 1/2, and HCV. Use UNIVERSAL PRECAUTIONS when handling. Human tissue derivatives must be treated as a potentially infectious agent and disposed of appropriately

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.