Human Breast Tissue Lysate (Adult Tumor) Summary
Human Breast Tumor Tissue Lysate, Soluble protein fraction
Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
These lysates are proteomic discovery tools.Researchers should validate and optimize for individual use. Potential applications MAY include WB, immunoprecipitation, protein-protein interactions, ligand binding, ELISA.
Note: For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required.
Packaging, Storage & Formulations
Store at -20C short term. Aliquot and store at -70C long term. Avoid freeze-thaw cycles.
Lysate Details for Array
The vial is provided with a 10% overfill. Maximum recovery can be obtained by centrifuging the vial briefly to collect any solution on the cap and tube sides.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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