Human Breast Tissue Lysate (Adult Normal)



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Human Breast Tissue Lysate (Adult Normal) Summary

Clinical Tissue
Human Breast Normal Tissue Lysate
Tissue: Breast
Diagnosis:Invasive ductal carcinoma
Sex: Female
Age :36
Gross findings: Tumor size 4 x 4 x 3 cm. Cut section hard and white/gray. Ipsilateral axillary lymph nodes examined: 7
Microscopic findings: Cancer in surgical margin of section: Negative
Multifocal carcinoma: Negative
Pectoral muscular/facia invasion: Negative
Chest wall invasion: Negative
Breast skin dermal invasion only: Negative
Skin invasion with ulceration: Negative
Dermal lymphatic channel invasion: Negative
Lymphatic and/or blood vessel invasion: Negative
Tumor necrosis: Positive
Number of nodes positive: 4/7
Tubule formation: 3
Nuclear pleomorphism: 3
Mitotic activity: 1
Lymphocytic response: Positive
Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.


Application Notes
These lysates have not been subjected to denaturing or reducing conditions. This allows the tissue or cell lysate to be used in a variety of applications; to study protein-protein interaction, ligand binding, ELISA, immunoprecipitation, 1D and 2D gel electrophoresis, and Western blotting for the detection of specific protein targets. For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required.
Buffer requirements for performing protein-protein interaction and ligand binding studies can vary significantly from RIPA buffer and may require modifications. In most cases, tissue lysates in RIPA buffer can be used, directly in standard ELISA and immunoprecipitation assays.
This material has tested negative for HbsAg, HIV 1/2, and HCV. Use UNIVERSAL PRECAUTIONS when handling. Human tissue derivatives must be treated as a potentially infectious agent and disposed of appropriately.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
1 mg/ml

Lysate Details for Array

Life Stage
Tissue Condition


The vial is provided with a 10% overfill. Maximum recovery can be obtained by centrifuging the vial briefly to collect any solution on the cap and tube sides.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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