BrdU Cell Proliferation Assay Kit

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Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Suitable Sample Type
Adherent and suspension cells

Order Details

BrdU Cell Proliferation Assay Kit Summary

Description
BrdU Cell Proliferation Assay Kit: Easy & Sensitive BrdU based Colorimetric Assay to Measure Cell Proliferation in response to growth factors, cytokines, mitogens and nutrients etc. Kit Size: 200 & 1000 Assays.

  • Detection method- fluorecence (400 nm excitation filter and 505 nm emission filter)
  • Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Kit Type
Assay Kit

Applications/Dilutions

Application Notes
5-bromo-2-deoxyuridine (BrdU) is a pyrimidine analog. It gets incorporated into the newly synthesized DNA of proliferating cells in place of thymidine. Novus's BrdU Cell Proliferation Assay Kit detects incorporated BrdU using a mouse anti-BrdU antibody. An anti-mouse HRP-linked secondary antibody is used to detect the anti-BrdU antibody bound to BrdU, which is followed by addition of TMB (a HRP substrate). The extent of color development is proportional to the quantity of BrdU incorporated into the cells and can be used directly as an indicator of cell proliferation. Compared to other cell proliferation assays, this kit detects only the proliferating cells and not the seeded cells. This highly sensitive, non-radioactive kit detects as less as 50-100 proliferating cells

Packaging, Storage & Formulations

Storage
Store at -20C.

Kit Components

Components
  1. BrdU (1000X)
  2. BrdU Detection Antibody (300X)
  3. Fixing/Denaturing Solution
  4. Anti-mouse HRP-linked Antibody (2000X)
  5. Antibody Diluent
  6. Wash Buffer (10X)
  7. TMB Substrate
  8. Stop Solution

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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