BrdU Cell Proliferation Assay Kit (Colorimetric)

• Reactivity: Hu, Mu, Rt, Ma
• Suitable Sample Type: Adherent and suspension cells

BrdU Cell Proliferation Assay Kit (Colorimetric) Summary

• Description: 5-bromo-2-deoxyuridine (BrdU) is a pyrimidine analog. It gets incorporated into the newly synthesized DNA of proliferating cells in place of thymidine. The BrdU Cell Proliferation Assay Kit detects incorporated BrdU using a mouse anti-BrdU antibody. An anti-mouse HRP-linked secondary antibody is used to detect the anti-BrdU antibody bound to BrdU, which is followed by addition of TMB (a HRP substrate). The extent of color development is proportional to the quantity of BrdU incorporated into the cells and can be used directly as an indicator of cell proliferation. Compared to other cell proliferation assays, this kit detects only the proliferating cells and not the seeded cells. This highly sensitive, non-radioactive kit detects as few as 50-100 proliferating cells.
  
  • Detection method: Absorbance at 450 nm
  • Applications: Detection and quantification of cell proliferation induced by growth factors, cytokines, mitogens, and nutrients. Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation.
• Kit Type: Assay Kit (Colorimetric)

Applications/Dilutions

Publications

Read Publications using NBP2-54888 in the following applications: CellProlif 1 publication In vivo 1 publication

Packaging, Storage & Formulations

• Storage: Store at -20C.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for BrdU Cell Proliferation Kit (NBP2-54888)(3)

Publications using NBP2-54888

• Raj Gupta Y, Senthilkumaran B Common carp pentraxin gene: Evidence for its role in ovarian differentiation and growth Gen. Comp. Endocrinol. Jan 22 2020 [PMID: 31981692] (CellProlif, Fish)
• Oliveira, SDS;Chen, J;Castellon, M;Mao, M;Usha Raj, J;Comhair, S;Erzurum, S;Silva, CLM;Machado, RF;Bonini, MG;Minshall, RD; Injury-Induced Shedding of Extracellular Vesicles Depletes Endothelial Cells of Cav-1 (Caveolin-1) and Enables TGF-beta (Transforming Growth Factor-beta)-Dependent Pulmonary Arterial Hypertension Arterioscler. Thromb. Vasc. Biol. Apr 4 2019 [PMID: 30943774] (In vivo, Human)
• McDaniel K, Wu N, Zhou T et al. Amelioration of Ductular Reaction by Stem Cell Derived Extracellular Vesicles in MDR2 knockout mice via let-7 microRNA Hepatology Feb 5 2019 [PMID: 30723922] (Mouse)