BrdU Cell Proliferation Assay Kit (Colorimetric)

Images

 
There are currently no images for BrdU Cell Proliferation Kit (NBP2-54888).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Suitable Sample Type
Adherent and suspension cells

Order Details

BrdU Cell Proliferation Assay Kit (Colorimetric) Summary

Description
5-bromo-2-deoxyuridine (BrdU) is a pyrimidine analog. It gets incorporated into the newly synthesized DNA of proliferating cells in place of thymidine. The BrdU Cell Proliferation Assay Kit detects incorporated BrdU using a mouse anti-BrdU antibody. An anti-mouse HRP-linked secondary antibody is used to detect the anti-BrdU antibody bound to BrdU, which is followed by addition of TMB (a HRP substrate). The extent of color development is proportional to the quantity of BrdU incorporated into the cells and can be used directly as an indicator of cell proliferation. Compared to other cell proliferation assays, this kit detects only the proliferating cells and not the seeded cells. This highly sensitive, non-radioactive kit detects as few as 50-100 proliferating cells.
  • Detection method: Absorbance at 450 nm
  • Applications: Detection and quantification of cell proliferation induced by growth factors, cytokines, mitogens, and nutrients. Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation.
Kit Type
Assay Kit (Colorimetric)

Packaging, Storage & Formulations

Storage
Store at -20C.

Kit Components

Components
  1. BrdU (1000X)
  2. BrdU Detection Antibody (300X)
  3. Anti-mouse HRP-linked Antibody (2000X)
  4. Antibody Diluent
  5. Fixing/Denaturing Solution
  6. Wash Buffer (10X)
  7. TMB Substrate
  8. Stop Solution

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for BrdU Cell Proliferation Kit (NBP2-54888) (0)

There are no publications for BrdU Cell Proliferation Kit (NBP2-54888).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for BrdU Cell Proliferation Kit (NBP2-54888) (0)

There are no reviews for BrdU Cell Proliferation Kit (NBP2-54888). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for BrdU Cell Proliferation Kit (NBP2-54888) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Array NBP2-54888

Blogs on BrdU Cell Proliferation

There are no specific blogs for BrdU Cell Proliferation, but you can read our latest blog posts.

Contact Information

Product PDFs

Review this Product

Be the first to review our BrdU Cell Proliferation Assay Kit (Colorimetric) and receive a gift card or discount.