Mouse Brain Whole Tissue Lysate (Adult Whole Normal)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Concentration
1 mg/ml

Order Details

Mouse Brain Whole Tissue Lysate (Adult Whole Normal) Summary

Specificity
Normal Mouse Brain whole cell extract lysate

Applications/Dilutions

Application Notes
Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOuT dissociating agents. Heat lysate - 95C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior - heating. The recommended loading volume per lane is 10-20 ul depending on the size format of your gel.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
1X SDS-PAGE Sample Buffer [62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8], 10% (v/v) Glycerol
Preservative
No Preservative
Concentration
1 mg/ml

Lysate Details for Array

Type
Tissue
Life Stage
Adult
Tissue
Nervous
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Background

This ready-to-use whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Brain Lysate (NB800-PC14). (Showing 1 - 1 of 1 FAQ).

  1. We are seeking a normal brain tissue lysate for control of Parkinson’s disease. In your website, there is Human Brain Parietal Lobe Whole Tissue Lysate (Adult Parkinson's) 1.0mg (NB820-59414). If you have any normal brain tissue lysate, please let me know.
    • Please see this link to all of our normal human brain tissue lysates.

Other Available Formats

NB800-PC14

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