| Immunogen | Synthetic peptide representing the BRAF V600E mutated amino acid sequence from amino acid 596 to 606 (GLATEKSRWSG). |
| Localization | Cytoplasm |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This BRAF V600E (Clone VE1) antibody is useful for Western blot and Immunohistochemistry-Paraffin applications. In WB, a target band for BRAF V600E mutated protein may be seen at its predicted molecular weight position of ~95 kD, only in the mutated tumors/cells. An additional band at ~75 kD may also be detected in the mutated samples which has been suggested as the splice variant of BRAF that includes the mutated kinase domain. For additional information / images on the validation of this antibody in IHC-P and WB application, the customer may follow the research publication by the inventor of this antibody: Capper et al. 2011 Acta Neuropathol 122(1):11-9. PMID: 21638088 |
| Theoretical MW | 95 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris (pH 7.5) and 0.3% carrier protein |
| Preservative | 0.1% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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