Bovine Serum Albumin Native Protein Summary
Native Bovine Serum Albumin.
Source: Bovine Serum
This product was prepared from normal serum by a multi-step process including selective precipitation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Bovine Serum.
| Protein/Peptide Type
- Western Blot 1:100 - 1:2000
- Fluorophore-linked immunosorbent assay 1:100 - 1:2000
- Immunofluorescence 1:10 - 1:500
Packaging, Storage & Formulations
Store at 4C in the dark.
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Polyethylene Glycol (PEG-8000)
0.01% Sodium Azide
Reconstitute with 1.0 ml deionized water.
For extended storage, aliquot reconstituted liquid and freeze at -20 C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 C as an undiluted liquid. Dilute only prior to immediate use.
Alternate Names for Bovine Serum Albumin Native Protein
- cell growth inhibiting protein 42
- growth-inhibiting protein 20
- serum albumin
Bovine Serum Albumin (BSA) is used for various biochemical applications including ELISA (Enzyme-Linked Immunosorbent Assay), high content screening assays, western blotting, FACS Buffer and immunohistochemistry. BSA as a blocking reagent is particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. Also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. Bovine Serum Albumin can also be used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 3 months from date of receipt.
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