Bovine Serum Albumin Native Protein

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Product Details

Summary
Reactivity BvSpecies Glossary
Applications WB, FLISA, IF
Conjugate
Biotin
Concentration
LYOPH

Order Details

Bovine Serum Albumin Native Protein Summary

Description
Native Bovine Serum Albumin protein.

Source: Bovine Serum

Preparation
Method
This product was prepared from normal serum delipidation a multi-stage process including selective precipitation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Bovine Albumin and anti-Bovine Serum.
Protein/Peptide Type
Native Protein
Gene
ALB
Purity
Immunoelectrophoresis (IEP)

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunofluorescence 1:10 - 1:500

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Polyethylene Glycol (PEG-8000)
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunoelectrophoresis (IEP)
Reconstitution Instructions
Reconstitute with 1.0 ml deionized water.

Notes

For extended storage, aliquot reconstituted liquid and freeze at -20 C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Bovine Serum Albumin Native Protein

  • albumin
  • BSA
  • cell growth inhibiting protein 42
  • DKFZp779N1935
  • growth-inhibiting protein 20
  • PRO0883
  • PRO0903
  • PRO1341
  • serum albumin

Background

Bovine Serum Albumin (BSA) is used for various biochemical applications including ELISA (Enzyme-Linked Immunosorbent Assay), high content screening assays, western blotting, FACS Buffer and immunohistochemistry. BSA as a blocking reagent is particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. Also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. Bovine Serum Albumin can also be used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol ALB