Bovine Rotavirus Antigen ELISA Kit (Colorimetric)

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Product Details

Summary
Applications ELISA
Suitable Sample Type
feces
Standard Curve Range
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Sensitivity
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Order Details

Bovine Rotavirus Antigen ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of bovine rotavirus antigen. No significant cross-reactivity or interference between bovine rotavirus antigen and analogues was observed.
Standard Curve Range
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Sensitivity
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Assay Type
Sandwich ELISA
Inter-Assay
%CV < 15
Intra-Assay
%CV < 15
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay plate
  3. HRP-conjugate
  4. Instruction manual
  5. Negative Control
  6. Positive Control
  7. Stop Solution
  8. Substrate A
  9. Substrate B
  10. Wash Buffer

Notes

Please contact our Technical Services with any questions regarding species reactivity

Background

This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to rotavirus antigen. Samples are pipetted into the wells with antibody conjugated Horseradish Peroxidase(HRP). Any rotavirus antigen present in samples will bind to the pre-coated specific antibody. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of bovine rotavirus antigen bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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