Measured by its ability to bind immobilized rmLOX-1 (Catalog # 1564-LX) in an ELISA type binding assay with an estimated KD <75 nM.
Bovine serum-derived AGE-BSA protein Advanced glycation end product (AGE) of bovine serum albumin (AGE-BSA) was prepared by incubating BSA and glucose under sterile conditions at 37 °C for 60 days and then biotinylated.
<0.10 EU per 1 μg of the protein by the LAL method.
Read Publication using BT4127 in the following applications:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 1 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Bovine AGE-BSA Biotinylated Protein, CF
Advanced glycation end products (AGEs) are formed by the non-enzymatic reaction of reducing sugars with amino groups on macromolecules (1, 2). Glycation is accomplished by the Maillard reaction, which is a multistep process that begins with Schiff base formation between the amine and the carbonyl group on the sugar followed by rearrangement to form Amadori intermediates. The intermediates are oxidized to highly reactive dicarbonyl compounds which target the primary amino groups in lysine and arginine residues (3). Some AGEs involve protein crosslinking, while others, such as N epsilon -carboxymethyl lysine (CML), are confined to single molecules (3). The final step of AGE formation is essentially irreversible, but the Schiff bases and Amadori intermediates are susceptible to degradation (4, 5). The Maillard reaction is accelerated under hyperglycemic and oxidative conditions. Renal failure, dietary AGE intake, and the normal aging process also contribute to the in vivo accumulation of AGEs (6 - 8). Increased AGE accumulation may be both a cause and effect of diabetes as well as multiple chronic inflammatory conditions such as Alzheimer’s disease, atherosclerosis, and arthritis (1, 2). AGEs can bind a variety of receptors, including RAGE, AGE-R1, -R2, -R3, and the scavenger receptors CD36, LOX-1, SR-AI, SR-AII, SR-BI, Stabilin-1/FEEL-1, and Stabilin-2/FEEL-2 (1). RAGE is a multiligand receptor that is upregulated on macrophages, monocytes, smooth muscle cells, and endothelial cells in response to AGE accumulation (9). RAGE activation induces a broad proinflammatory response (1, 2, 10). The increased production of reactive oxygen species promotes additional AGE formation and RAGE upregulation, a cycle that exacerbates diabetic complications and inflammation-induced tissue injury (1, 2).
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Ramasamy, R. et al. (2005) Glycobiology 15:16R.
Reddy, V.P. and A. Beyaz (2006) Drug Discov. Today 11:646.
Szwergold, B.S. et al. (2001) Diabetes 50:2139.
Szwergold, B.S. et al. (2005) Ann. N.Y. Acad. Sci. 1043:845.
Thornalley, P.J. (2006) J. Ren. Nutr. 16:178.
Chuyen, N. et al. (2006) Mol. Nutr. Food Res. 50:1140.
Yin, D. and K. Chen (2005) Exp. Gerontol. 40:455.
Bierhaus, A, et al. (2006) Curr. Opin. Investig. Drugs 7:985.
Kislinger, T. et al. (1999) J. Biol. Chem. 274:31740.
Publications for AGE-BSA (BT4127)(1)
We have publications tested in 1 confirmed species: Mouse.
We have publications tested in 1 application: Bioassay.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.