Borrelia ELISA Kit (Colorimetric) Summary
| Description |
Borrelia IgM ELISA Kit is a solid phase enzyme immunoassay for the quantitative measurement of IgM autoantibodies against Borrelia. |
| Sensitivity |
2.41 AU/mL |
| Assay Type |
solid phase |
| Inter-Assay |
CV% < 6.55% |
| Intra-Assay |
CV% < 2.55% |
| Spike Recovery |
92% to 107% |
| Sample Volume |
100 uL (1:100 for serum ; 1:1 for CSF or 1:80 Synovial Fluid for prediluted) |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
Kit Components
|
Components
|
- 1) r.t.u. = ready to use
- 2) The antibody concentration for each Standard (A-E) is mentioned in enclosed Quality control certificateAU/mL (Artificial units/mL).
- Anti-human IgM antibodies labeled with horseradish peroxidase (Px-conjugate) r.t.u.
- Chromogenic substrate (TMB substrate), r.t.u.
- Dilution buffer anti-Borrelia rec., r.t.u.
- ELISA break-away strips coated with specific recombinant antigens
- Standard A = negative control serum, r.t.u.1), 2)
- Standard B, r.t.u.
- Standard C, r.t.u.
- Standard D = calibrator, r.t.u.
- Standard E = positive control serum, r.t.u.
- Stop solution, r.t.u.
- Wash buffer concentrate, 10x concentrated.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Background
Borrelia burgdorferi is a spirochete and the cause of Lyme disease, a tick transmitted illness of humans and animals. B. burgdorferi may persist in humans and animals for months or years following initial infection, despite a robust humoral immune response. B. burgdorferi resembles other spirochetes in that it is a highly specialized, motile, two-membrane, spiral shaped bacteria which lives primarily as an extracellular pathogen. B. burgdorferi has an unusual genome compared with other eubacteria which includes a linear chromosome approximately one megabase in size and numerous linear and circular plasmids.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Borrelia ELISA Kit (KA1468). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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