Bordetella Pertussis Toxin Antibody (1280/204) [Alexa Fluor™ Plus 488]

• Reactivity: Ba
• Applications: ELISA
• Clone: 1280/204
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 488

Bordetella Pertussis Toxin Antibody (1280/204) [Alexa Fluor™ Plus 488] Summary

• Immunogen: Native Bordatella pertussis toxin
• Localization: Secreted
• Specificity: NB100-64514 is reactive with Bordetella pertussis toxin in a simple ELISA. Reactivities with other organisms have not yet been determined.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Bordetella Pertussis Toxin Antibody (1280/204) [Alexa Fluor™ Plus 488]

• pertussis toxin A protomer
• pertussis toxin

Background

Pertussis toxin (islet-activating protein) is the major protein toxin produced by virulent strains of Bordetella pertussis, the organism that causes whooping cough. As revealed by polyacrylamide gel electrophoresis, the purified protein consists of five dissimilar subunits: S1 (MW 28,000), S2 (MW 23,000), S3 (MW 22,000), S4 (MW 11,700) and S5 (MW 9,300), in a molar ratio of 1:1:1:2:1. The A-protomer, S1 is responsible for the enzymatic activity of the toxin. Together, S2, S3, S4 and S5 comprise the B-oligomer, responsible for binding the toxin to the cell surface.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.