Blu10 Plus Prestained Protein Ladder

Western Blot: Blu10 Plus Prestained Protein Ladder [NBP3-33170] - The Blu10 Plus Prestained Protein Ladder is a combination of 10 pre-stained proteins with molecular weights from 6.5 to 270 kDa. The 10 recombinant proteins are covalently coupled with blue chromophore, while 2 orange bands at 30 kDa and 270 kDa and a green band at 52 kDa serve as reference bands. The Blu10 Plus Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

• Applications: WB

Blu10 Plus Prestained Protein Ladder Summary

• Description: The Blu10 Plus Prestained Protein Ladder is a combination of 10 pre-stained proteins with molecular weights from 6.5 to 270 kDa. The 10 recombinant proteins are covalently coupled with blue chromophore, while 2 orange bands at 30 kDa and 270 kDa and a green band at 52 kDa serve as reference bands. The Blu10 Plus Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes..
  
  (10 pre-stained bands, 6.5-270 kDa)

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: 3 ul or 5 ul per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 ul per well for general Western transferring.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 62.5 mM Tris-H3PO4 (pH 7.5), 1 mM EDTA, 2% SDS, 10 mM DTT, 33% glycerol

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.