Blank Comp-Bead Particles


Blank Comp-Bead Particles [NBP3-00500] - Coulter M3 analysis: Mean particle size 3.35 micron.
Blank Comp-Bead Particles [NBP3-00500] - Histograms showing low fluorescence in the Red, Green, and Orange channels.

Product Details

Applications Flow

Order Details

Blank Comp-Bead Particles Summary

The Blank Compensation Beads serve as a negative control for setting compensation in multicolor flow cytometry.

Concentration: 10^7 particles / mL

Particle size: 3.0 -3.4 micron

Particle material: polystyrene


  • Flow Cytometry
Application Notes
Resuspend by vortexing before use. To achieve optimum particle suspension, sonicate the reagent after vortex mixing.

Packaging, Storage & Formulations

Store at 4C in the dark. Do not freeze.
0.016 M PBS (pH 7.4), 0.2% BSA
0.02% Sodium Azide

Alternate Names for Blank Comp-Bead Particles

  • Comp Beads
  • Compensation Beads
  • Flow Cytometry Compensation
  • Negative Compensation Beads


Compensation is the mathematical correction for the amount of overlap (spillover) of one fluorochrome's emission spectra into another fluorochrome's channel. To calculate this overlap, record single-color stained controls for each fluorochrome in a multicolor panel. This allows the cytometer to visualize the emission of each fluorochrome in each channel, or detector, and subtract the spillover, generating a matrix to apply to multi-color experimental samples. To ensure accurate calculations, single-color controls need to be as bright, or brighter, than the marker on the cell of interest. With increased use of multi-parametric flow cytometry and the large number of fluorochromes available, it is more important than ever to ensure accurate compensation for reliable interpretation of data and reproducible experimental results.

Compensation beads are a highly desirable alternative to traditional cell-based single-color compensation. Positive beads can come pre-loaded with anti-IgG for conjugated antibody capture of mouse, rat, or hamster origin. Uncoated (negative) beads ensure standardization of the autofluorescence in each channel. Beads are preferable to cell-based compensation for several reasons:

1) No precious sample is wasted, and more sample can be used for experimental acquisition.

2) Since 100% of positive beads are able to capture the antibody, the exact experimental fluorochrome can be used regardless of a marker's cellular expression. Dimly expressed markers may not be bright enough for compensation using cells.

3) Reduced autofluorescence of beads allows for more precise calculation of spectral overlap.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for Blank Comp-Bead Particles (NBP3-00500) (0)

There are no publications for Blank Comp-Bead Particles (NBP3-00500).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Blank Comp-Bead Particles (NBP3-00500) (0)

There are no reviews for Blank Comp-Bead Particles (NBP3-00500). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Blank Comp-Bead Particles (NBP3-00500) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Blank Comp-Bead Particles Products

Blogs on Blank Comp-Bead Particles

There are no specific blogs for Blank Comp-Bead Particles, but you can read our latest blog posts.

Contact Information

Product PDFs

Review this Product

Be the first to review our Blank Comp-Bead Particles and receive a gift card or discount.