Western Blot: BirA Antibody (5B11c3-3) [NBP2-59939] - Analysis in total cell lysate of parental HF (lane 1) or HF stably expressing GFP-NLS-BirA without (lane 2) or with induced expression (lane 3) were transferred to a ...read more
Immunocytochemistry/ Immunofluorescence: BirA Antibody (5B11c3-3) [NBP2-59939] - HeLa cells transfected with red fluorescent protein fused to BirA amino acid residues 151-321 (RFP-BirA 151-321) were fixed with 4% PFA ...read more
Immunocytochemistry/ Immunofluorescence: BirA Antibody (5B11c3-3) [NBP2-59939] - Shown are confocal images of human fibroblasts (HF) expressing green fluorescence protein (GFP) fused to a nuclear localization signal and ...read more
Immunocytochemistry/ Immunofluorescence: BirA Antibody (5B11c3-3) [NBP2-59939] - HEK293 stably expressing BirA tagged to a nuclear envelope protein SLAP75 (BirA-SLAP75) were fixed in cold methanol and stained with ...read more
This antibody recognises the C-terminal half residues 151-321 of E.coli BirA. It has not been tested on other species.
35 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBP2-59939 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Protein A purified
Positive Control: Cells overexpressing an E.coli BirA construct
Alternate Names for BirA Antibody (5B11c3-3)
Bifunctional protein birA
Biotin [acetyl CoA carboxylase] synthetase
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for BirA Antibody (NBP2-59939)(2)
We have publications tested in 1 confirmed species: E. coli.
We have publications tested in 1 application: WB.
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Publications using NBP2-59939
Klein S, Stern D, Seeber F Expression of in vivo biotinylated recombinant antigens SAG1 and SAG2A from Toxoplasma gondii for improved seroepidemiological bead-based multiplex assays Research Square Jun 9 2020 (WB, E. coli)
Antibody diluted 1:1000. Blots came out clean. The cells were HEK-293 transfected with either pcDNA3.1(+) empty vector as a negative control or the pcDNA vector expressing the HIV-1 Vpu fused to BirA.
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