BirA Antibody (5B11c3-3)

Images

 
Western Blot: BirA Antibody (5B11c3-3) [NBP2-59939] - Analysis in total cell lysate of parental HF (lane 1) or HF stably expressing GFP-NLS-BirA without (lane 2) or with induced expression (lane 3) were transferred to a ...read more
Immunocytochemistry/ Immunofluorescence: BirA Antibody (5B11c3-3) [NBP2-59939] - HeLa cells transfected with red fluorescent protein fused to BirA amino acid residues 151-321 (RFP-BirA 151-321) were fixed with 4% PFA ...read more
Immunocytochemistry/ Immunofluorescence: BirA Antibody (5B11c3-3) [NBP2-59939] - Shown are confocal images of human fibroblasts (HF) expressing green fluorescence protein (GFP) fused to a nuclear localization signal and ...read more
Immunocytochemistry/ Immunofluorescence: BirA Antibody (5B11c3-3) [NBP2-59939] - HEK293 stably expressing BirA tagged to a nuclear envelope protein SLAP75 (BirA-SLAP75) were fixed in cold methanol and stained with ...read more

Product Details

Summary
Reactivity EcSpecies Glossary
Applications WB, ICC/IF
Clone
5B11c3-3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

BirA Antibody (5B11c3-3) Summary

Immunogen
GST fused to E.coli BirA R118G
Specificity
The antibody recognises the C-terminal half of E.coli BirA; the antibody recognised the BirA variant miniTurbo; not tested for TurboID or other BirA derivate.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1-2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
Application Notes
This antibody recognises the C-terminal half residues 151-321 of E.coli BirA. It has not been tested on other species.
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-59939 in the following applications:

Publications
Read Publications using
NBP2-59939 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Notes

Positive Control: Cells overexpressing an E.coli BirA construct

Alternate Names for BirA Antibody (5B11c3-3)

  • Bifunctional protein birA
  • Bio R
  • bioR
  • Biotin [acetyl CoA carboxylase] synthetase
  • BirA
  • dhbB
  • ECK3965

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BirA Antibody (NBP2-59939)(2)

We have publications tested in 1 confirmed species: E. coli.

We have publications tested in 1 application: WB.


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WB
(1)
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E. coli
(1)
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Showing Publications 1 - 2 of 2.
Publications using NBP2-59939 Applications Species
Klein S, Stern D, Seeber F Expression of in vivo biotinylated recombinant antigens SAG1 and SAG2A from Toxoplasma gondii for improved seroepidemiological bead-based multiplex assays Research Square Jun 9 2020 (WB, E. coli) WB E. coli
Creed TM, Baldeosingh R, Eberly CL et al. The PAX-SIX-EYA-DACH network modulates GATA-FOG function in fly hematopoiesis and human erythropoiesis Development Jan 3 2020 [PMID: 31806659]

Review for BirA Antibody (NBP2-59939) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-59939:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot BirA NBP2-59939
Enlarge
5
reviewed by:
Wyatt Henke
WB Human 05/08/2020
View

Summary

ApplicationWestern Blot
Sample Tested293 whole cell lysate
SpeciesHuman
LotCRT/17/131

Comments

CommentsAntibody diluted 1:1000. Blots came out clean. The cells were HEK-293 transfected with either pcDNA3.1(+) empty vector as a negative control or the pcDNA vector expressing the HIV-1 Vpu fused to BirA.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BirA Antibody (NBP2-59939). (Showing 1 - 1 of 1 FAQs).

  1. What's the differences between BirA Antibody (5B11c3-3) NBP2-59938 and BirA Antibody (6C4c7) NBP2-59939?
    • They are very similar, just different clones raised against the same immunogen. They both work well and are guaranteed for WB and ICC/IF.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-59939AF350
Alexa Fluor 405 NBP2-59939AF405
Alexa Fluor 488 NBP2-59939AF488
Alexa Fluor 532 NBP2-59939AF532
Alexa Fluor 594 NBP2-59939AF594
Alexa Fluor 647 NBP2-59939AF647
Alexa Fluor 700 NBP2-59939AF700
Alexa Fluor 750 NBP2-59939AF750
Biotin NBP2-59939B
DyLight 350 NBP2-59939UV
DyLight 405 NBP2-59939V
DyLight 488 NBP2-59939G
DyLight 550 NBP2-59939R
DyLight 594 NBP2-59939DL594
DyLight 650 NBP2-59939C
DyLight 680 NBP2-59939FR
DyLight 755 NBP2-59939IR
FITC NBP2-59939F
HRP NBP2-59939H
Janelia Fluor 549 NBP2-59939JF549
Janelia Fluor 646 NBP2-59939JF646

Additional BirA Products

Blogs on BirA

There are no specific blogs for BirA, but you can read our latest blog posts.
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Calculators

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Recent Reviews

5
5
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1
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Wyatt Henke
05/08/2020
Application: WB
Species: Human