Bovine Beta Lactoglobulin ELISA Kit (Colorimetric) Summary
This assay has high sensitivity and excellent specificity for detection of bovine LGB. No significant cross-reactivity or interference between bovine LGB and analogues was observed.
|Standard Curve Range
0.156 ug/ml-10 ug/ml
CV% < 10%
CV% < 8%
94% (EDTA plasma)
ELISA Kit (Colorimetric)
Packaging, Storage & Formulations
- Stop Solution
- Standard (Freeze dried)
- Sample Diluent
- TMB Substrate
- Wash Buffer (25 x concentrate)
- Assay plate
- Biotin-conjugate (100 x concentrate)
- Biotin-conjugate Diluent
- Adhesive Strip (For 96 wells)
- HRP-avidin (100 x concentrate)
- HRP-avidin Diluent
- Instruction manual
Please contact our Technical Services with any questions regarding species reactivity
This assay employs the competitive inhibition enzyme immunoassay technique. Antibody specific for LGB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated LGB. A competitive inhibition reaction is launched between LGB (Standards or samples) and biotin-conjugated LGB with the pre-coated LGB antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of LGB bound in the initial step. The color development is stopped and the intensity of the color is measured.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed
for 6 months from date of receipt.
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