Bovine Beta Lactoglobulin ELISA Kit (Colorimetric)

ELISA: Bovine Beta Lactoglobulin ELISA Kit [NBP2-59955] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Bovine Beta Lactoglobulin ELISA Kit (Colorimetric) [NBP2-59955] - Standard Curve Reference

• Reactivity: Bv
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and milk
• Standard Curve Range: 0.156 ug/mL - 10 ug/mL
• Sensitivity: 0.129 ug/mL

Bovine Beta Lactoglobulin ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of bovine LGB. No significant cross-reactivity or interference between bovine LGB and analogues was observed.
• Standard Curve Range: 0.156 ug/mL - 10 ug/mL
• Sensitivity: 0.129 ug/mL
• Assay Type: Competitive ELISA
• Inter-Assay: %CV < 10
• Intra-Assay: %CV < 8
• Spike Recovery: 89 - 100%
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Background

This assay employs the competitive inhibition enzyme immunoassay technique. Antibody specific for LGB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated LGB. A competitive inhibition reaction is launched between LGB (Standards or samples) and biotin-conjugated LGB with the pre-coated LGB antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of LGB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.