Bovine Beta Lactoglobulin ELISA Kit (Colorimetric)

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ELISA: Bovine Beta Lactoglobulin ELISA Kit [NBP2-59955] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity BvSpecies Glossary
Applications ELISA
Suitable Sample Type
serum, plasma and milk
Standard Curve Range
0.156 ug/ml-10 ug/ml
Sensitivity
0.129 ug/ml

Order Details

Bovine Beta Lactoglobulin ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of bovine LGB. No significant cross-reactivity or interference between bovine LGB and analogues was observed.
Standard Curve Range
0.156 ug/ml-10 ug/ml
Sensitivity
0.129 ug/ml
Assay Type
Competitive ELISA
Inter-Assay
CV% < 10%
Intra-Assay
CV% < 8%
Spike Recovery
96% (serum) 94% (EDTA plasma)
Sample Volume
50-100ul
Kit Type
ELISA Kit (Colorimetric)

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Stop Solution
  2. Standard (Freeze dried)
  3. Sample Diluent
  4. TMB Substrate
  5. Wash Buffer (25 x concentrate)
  6. Assay plate
  7. Biotin-conjugate (100 x concentrate)
  8. Biotin-conjugate Diluent
  9. Adhesive Strip (For 96 wells)
  10. HRP-avidin (100 x concentrate)
  11. HRP-avidin Diluent
  12. Instruction manual

Notes

Please contact our Technical Services with any questions regarding species reactivity

Background

This assay employs the competitive inhibition enzyme immunoassay technique. Antibody specific for LGB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated LGB. A competitive inhibition reaction is launched between LGB (Standards or samples) and biotin-conjugated LGB with the pre-coated LGB antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of LGB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Array NBP2-59955

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