Recombinant E. coli beta-lactamase Protein

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Product Details

Summary
Reactivity EcSpecies Glossary
Applications Bioactivity
Concentration
LYOPH

Order Details

Recombinant E. coli beta-lactamase Protein Summary

Description
A single non-glycosylated polypeptide chain containing 264 amino acids.

Source: Escherichia coli.

Amino Acid Sequence:
MHPETLVKVK DAEDQLGARV GYIELDLNSG KILESFRPEE RFPMMSTFKV LLCGAVLSRV DAGQEQLGRR IHYSQNDLVE YSPVTEKHLT DGMTVRELCS AAITMSDNTA ANLLLTTIGG PKELTAFLHN MGDHVTRLDR WEPELNEAIP NDERDTTMPA AMATTLRKLL TGELLTLASR QQLIDWMEAD KVAGPLLRSA LPAGWFIADK SGAGERGSRG IIAALGPDGK PSRIVVIYTT GSQATMDERN RQIAEIGASL IKHW

Details of Functionality
Fully biologically active when compared to standard. One unit of enzyme activity is defined as the amount of enzyme which will hydrolyze 1.0 umol of benzyl penicillin in presence of EDTA at pH 7.0 and at 25 C.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
ampC
Purity
>95%, by SDS-PAGE
Endotoxin Note
Less than 0.1 EU/ug of rPPase as determined by LAL method.

Applications/Dilutions

Theoretical MW
28.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 100 mM Tris, pH 7.0.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant E. coli beta-lactamase Protein

  • ampA
  • AmpC
  • cephalosporinase
  • EC 3.5.2.6
  • ECK4146

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Other Available Formats

Additional beta-lactamase Products

Array NBP2-61444

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Bioinformatics

Gene Symbol ampC
Uniprot