Recombinant E. coli beta-lactamase Protein

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SDS-Page: Recombinant E. coli Beta Lactamase Protein [NBP2-59641] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity EcSpecies Glossary
Applications Func, PAGE, Bioactivity
Concentration
1 mg/ml

Order Details

Recombinant E. coli beta-lactamase Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 20-377 of E. coli Beta Lactamase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAPQQINDIV HRTITPLIEQ QKIPGMAVAV IYQGKPYYFT WGYADIAKKQ PVTQQTLFEL GSVSKTFTGV LGGDAIARGE IKLSDPTTKY WPELTAKQWN GITLLHLATY TAGGLPLQVP DEVKSSSDLL RFYQNWQPAW APGTQRLYAN SSIGLFGALA VKPSGLSFEQ AMQTRVFQPL KLNHTWINVP PAEEKNYAWG YREGKAVHVS PGALDAEAYG VKSTIEDMAR WVQSNLKPLD INEKTLQQGI QLAQSRYWQT GDMYQGLGWE MLDWPVNPDS IINGSDNKIA LAARPVKAIT PPTPAVRASW VHKTGATGGF GSYVAFIPEK ELGIVMLANK NYPNPARVDA AWQILNALQ

Details of Functionality
Specific activity is >700 units/mg, in which One unit will hydrolyze 1.0umole of Nitrocefin per minute at pH 7.0 at 37C.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
ampC
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
41.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant E. coli beta-lactamase Protein

  • ampA
  • AmpC
  • cephalosporinase
  • EC 3.5.2.6
  • ECK4146

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Other Available Formats

Additional beta-lactamase Products

Array NBP2-59641

Bioinformatics Tool for beta-lactamase Recombinant Protein (NBP2-59641)

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Bioinformatics

Gene Symbol ampC