Recombinant E. coli beta-lactamase His Protein

SDS-Page: Recombinant E. coli Beta Lactamase Protein [NBP1-72284] - 15% SDS-PAGE (3 ug)

• Reactivity: Ec
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant E. coli beta-lactamase His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 20-377 of E.coli Beta Lactamase
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAPQQINDIV HRTITPLIEQ QKIPGMAVAV IYQGKPYYFT WGYADIAKKQ PVTQQTLFEL GSVSKTFTGV LGGDAIARGE IKLSDPTTKY WPELTAKQWN GITLLHLATY TAGGLPLQVP DEVKSSSDLL RFYQNWQPAW APGTQRLYAN SSIGLFGALA VKPSGLSFEQ AMQTRVFQPL KLNHTWINVP PAEEKNYAWG YREGKAVHVS PGALDAEAYG VKSTIEDMAR WVQSNLKPLD INEKTLQQGI QLAQSRYWQT GDMYQGLGWE MLDWPVNPDS IINGSDNKIA LAARPVKAIT PPTPAVRASW VHKTGATGGF GSYVAFIPEK ELGIVMLANK NYPNPARVDA AWQILNALQ

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: ampC
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 41.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant E. coli beta-lactamase His Protein

• ampA
• AmpC
• cephalosporinase
• EC 3.5.2.6
• ECK4146

Background

AmpC, also known as Beta-lactamase, is the most widespread resistance mechanism to beta-lactam antibiotics, such as the penicillins and the cephalosporins. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties. Recombinant E.coli beta-lactamase protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for beta-lactamase Recombinant Protein (NBP1-72284). (Showing 1 - 1 of 1 FAQ).

1. What kind of beta-lactamase does this antibody belong to? A, B, C or D? And what's molecular weight of beta-lactamase? Is it in already in solution or just solid?
   • This antibody recognizes Beta-Lactamase, class C. The molecular weight is 41.8 kDa (379aa) confirmed by MALDI-TOF. The sequence falls between 20-377aa of UniProt P00811 (https://www.uniprot.org/uniprotkb/P00811/entry). Protein accession number: NP_418574. This product is sold in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol at 1.0mg/ml.

Bioinformatics

• Gene Symbol: ampC